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啤酒污染菌的鉴定及其细胞膜脂肪酸的组成分析

邱然 陆健

食品与发酵工业2017,Vol.43Issue(7):55-61,7.
食品与发酵工业2017,Vol.43Issue(7):55-61,7.DOI:10.13995/j.cnki.11-1802/ts.013105

啤酒污染菌的鉴定及其细胞膜脂肪酸的组成分析

Identification of beer-spoilage bacterium and analysis of its membrane fatty acids composition

邱然 1陆健1

作者信息

  • 1. 江南大学生物工程学院,工业生物技术教育部重点实验室,粮食发酵工艺与技术国家工程实验室,江苏无锡,214122
  • 折叠

摘要

Abstract

A total of 11 strains of beer-spoilage bacteria were isolated from draft beer.The homology of 16S rRNA was analyzed and phylogenetic tree was structured for these 11 beer-spoilage bacteria.These 11 beer-spoilage bacteria were all classified into Lactobacillus genus,including Lactobacillus plantarum,Lactobacillus parabuchneri and Lactobacillus brevis.Variation of beer-spoilage bacteria membrane lipid composition was consistent with the result of 16S rRNA.The contents of straight chain unsaturated fatty acids and C18 and C20 fatty acids of BN01,BN02 and BN03 were increased with increase of beer content.These results not only classified and identified beer-spoilage bacteria but also expound tolerance mechanism of beer environmental stress based on regulation of fatty acids of beer bacteria membrane.

关键词

啤酒/啤酒污染菌/乳酸杆菌/鉴定/脂肪酸

Key words

beer/beer-spoilage bacteria/Lactobacillus/identification/membrane fatty acids

引用本文复制引用

邱然,陆健..啤酒污染菌的鉴定及其细胞膜脂肪酸的组成分析[J].食品与发酵工业,2017,43(7):55-61,7.

基金项目

863计划(啤酒用新酶创制与低碳制造关键技术研究2013AA102109) (啤酒用新酶创制与低碳制造关键技术研究2013AA102109)

江苏高校优势学科建设工程项目 ()

食品与发酵工业

OA北大核心CSCDCSTPCD

0253-990X

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