食品与发酵工业2017，Vol.43Issue(7)：214-221,8.DOI:10.13995/j.cnki.11-1802/ts.013441
不同种荞麦发芽前后蛋白质及氨基酸变化主成分分析与综合评价
Principal component analysis and comprehensive evaluation of protein and amino acid in different varieties of buckwheat and buckwheat sprout
摘要
Abstract
In order to evaluate the comprehensive qualities of protein and amino acids in different varieties of buckwheat and their sprouts,and study the effects of germination on protein and amino acid of buckwheat,the protein and amino acids content of 10 varieties of buckwheat (5 kinds of sweet buckwheat,5 kinds of tartarian buckwheat) and their sprouts were determined by Kjeldahl Nitrogen Determination and automatic amino acid analyzer.The correlations were analyzed by principal component analysis and systematic cluster analysis.The results showed that buckwheat seeds and their sprouts were rich in glutamic acid,arginine,lysine,alanine and aspartate amino acids.Germination process can significantly improve protein content (average growth of 50.0％),total amino acid (average growth of 37.0％),total essential amino acid (average growth of 43.7％),and most of amino acids.Three principal components were extracted,and principal component analysis of amino acids of buckwheat and comprehensive evaluation model was established,which is F =F1 × 0.766 1 + F2 × 0.103 4 + F3 × 0.066 6.The comprehensive scores of amino acids were calculated by the model for the overall quality of amino acids of buckwheat.The correlation coefficient between the composite scores and the content of total amino acids and essential amino acid were 0.974 1 and 0.990 9 respectively.Combining with clustering analysis,comprehensive evaluation results showed that the sprouts of Chifeng 2,Haizige tartarian buckwheat and Hunan 7-2 had a better quality.关键词
荞麦/发芽/氨基酸/主成分分析/综合评价Key words
buckwheat/germination/amino acids/principal component analysis/comprehensive evaluation引用本文复制引用
张雨薇,景梦琳,李小平,魏思凡,胡新中,马蓁,刘柳..不同种荞麦发芽前后蛋白质及氨基酸变化主成分分析与综合评价[J].食品与发酵工业,2017,43(7):214-221,8.基金项目
国家自然科学基金(31470093) （31470093）
陕西省自然科学基础研究计划(2015JM3109) （2015JM3109）
