中国食品添加剂Issue(7)：49-55,7.
田口方法优化菊糖源低聚果糖单体标准品制备工艺的研究
Optimization of inulin-derived oligofructose standard monomer using Taguchi method
摘要
Abstract
On the current Fos standard products market,it only has GFn oligomer standard products but lack of Fn oligomer standard products.The separation conditions for Fn oligofructose monomer from fructooligosaccharides mixtures derived from inulooligosaccharides were optimized by the Taguchi method in this study.The results showed that the sample concentration was the most significant factor for inulin separation.The optimum separation could be obtained at flow rate 0.05 mL/min,sample concentration 100 g/L,sample volume 2 mL and elution solution of 5％ ethanol with the column of Bio-Gel-P4 (2.0 × 100 cm).The sample then submitted to Bio-Gel-P2 (2.0 × 100 cm) column chromatography,the optimum separation conditions were:flow rate 0.05 mL/min,sample concentration 100 g/L,sample volume 1 mL and elution solution of 5％ ethanol.Under the above optimum conditions,the product from Bio-Gel-P4 column was concentrated and separated by Bio-Gel-P2 column chromatography.The purity of F3 was ≥ 98％,and F4 was ≥ 90％.关键词
菊糖/低聚果糖/分离/田口方法/优化Key words
inulin/oligosaccharide/separation/Taguchi method/optimization分类
轻工纺织引用本文复制引用
武敏,秦松,李莉莉,常秀莲..田口方法优化菊糖源低聚果糖单体标准品制备工艺的研究[J].中国食品添加剂,2017,(7):49-55,7.基金项目
国家自然科学基金(21306221) （21306221）
山东省科技重大专项(重大关键技术)(2015ZDJS03002) （重大关键技术）
烟台大学研究生科技创新基金资助(YDYB1716). （YDYB1716）
