中国食品添加剂Issue(7)：62-67,6.
香蓼总黄酮提取液提取条件及其体外抗氧化活性考察评价
Study on extraction conditions and antioxidant activity in vitro of total flavonoids extract from polygonum viscosum
摘要
Abstract
To optimize the extraction process of total flavonoids extract in polygonum viscosum and its in vitro antioxidant activity.The optimum technological parameters of extraction for total flavonoids extract from polygonum viscosum were determined by the orthogonal experiment on the basis of single factor experiment.The antioxidant activity was evaluated though detecting DPpH· scavenging rate and reducing activity test.The results showed that optimal technological factors for total flavonoids extract from polygonum viscosum were as follows:extraction temperature was 60 ℃,ethanol concentration was 70％ (v/v),solid to liquid was 1 ∶ 20,extraction time was 15 min,and extraction times were 2 times.Under the optimal conditions,it was found that the total flavonoids content in the polygonum viscosum was 145.0 ± 0.2 mg/g,average recovery rate of total flavonoids in the polygonum viscosum was 99.78％,variation coefficient was 1.00％ (n=5).The results of in vitro antioxidant test indicated that the antioxidant activity of total flavonoids extract in the polygonum viscosum was the same as tertiary butylhydroquinone (TBHQ),and the EC50 value (0.022 mg/mL) of DPPH· scavenging rate from polygonum viscosum was the same as the EC50 value (0.015 mg/mL) of TBHQ,but was much less than other Chinese medicinal herbs.Analysis results show that the total flavonoids ofpolygonum viscosum have stronger antioxidant activity and is a natural antioxidant agent.关键词
香蓼/总黄酮/抗氧化活性Key words
polygonum viscosum/total flavonoids/antioxidant activity分类
轻工纺织引用本文复制引用
苟体忠,常飞,王翔,杨昌军..香蓼总黄酮提取液提取条件及其体外抗氧化活性考察评价[J].中国食品添加剂,2017,(7):62-67,6.基金项目
国家青年自科基金(41303016) （41303016）
贵州省教育厅优秀人才项目(黔教合KY字[2012]099号) （黔教合KY字[2012]099号）
贵州省教育厅“高层次人才引进项目”(院科通[2012]7号) （院科通[2012]7号）
凯里学院分析化学重点学科(院通字[2014]157号) （院通字[2014]157号）
贵州省教育质量提升工程项目(黔教合KY字[2014] 228号) （黔教合KY字[2014] 228号）
凯里学院博士启动基金(BS201503). （BS201503）
