肉类研究2017,Vol.31Issue(8):6-11,6.DOI:10.7506/rlyj1001-8123-201708002
诺邓火腿粗肽抗氧化活性及亚硝酸钠清除活性
Antioxidant and Sodium Nitrite Scavenging Activity of Crude Peptide Extracted from Nuodeng Ham
摘要
Abstract
In this experiment, the scavenging capacities against hydroxyl and 2,2-diphenyl-1-picrylhydr-azyl (DPPH) free radicals, ferrous ion chelating activity, lipid oxidation inhibitory activity, and sodium nitrite scavenging capability under normal and simulated gastric conditions of the crude peptide extracted from Nuodeng ham were investigated. Results showed that the percentage scavenging of hydroxyl and DPPH free radicals by the crude peptide at 5.0 mg/mL was 47.81% and 67.10%, respectively, highly significantly different from that by glutathione (GSH) at the same concentration (P < 0.01). The percentage chelating of ferrous ion by the crude peptide at 5.0 mg/mL was 31.38%, not significantly different from that by GSH at the same concentration (P > 0.05). The percentage inhibition of lipid oxidation by the crude peptide at 25.0 mg/mL at 144 h of reaction was as high as 77.86%, highly significantly different from that by glutathione (GSH) at the same concentration (P < 0.01). Under normal and simulated gastric conditions, the percentage scavenging of sodium nitrite by the crude peptide at 10.0 mg/mL was (84.31 ± 0.77)% and (80.97 ± 2.00)%, respectively, highly significantly different from that by glutathione (GSH) at the same concentration (P < 0.01). Collectively, we concluded that the crude peptide extracted from Nuodeng ham had antioxidant and lipid oxidation inhibitory activities, and also exhibited strong sodium nitrite scavenging capacity.关键词
诺邓火腿/粗肽/抗氧化活性/亚硝酸钠清除活性Key words
Nuodeng ham/crude peptide/antioxidant activity/sodium nitrite scavenging capacity分类
轻工纺织引用本文复制引用
吴宝森,谷大海,王桂瑛,程志斌,徐志强,普岳红,汪善荣,廖国周..诺邓火腿粗肽抗氧化活性及亚硝酸钠清除活性[J].肉类研究,2017,31(8):6-11,6.基金项目
云南省现代农业(生猪)产业技术体系建设专项(2017KJTX0013) (生猪)
昆明市畜产品加工与质量控制重点实验室项目(2015-1-A-00563) (2015-1-A-00563)
公益性行业(农业)科研专项(201303082) (农业)