食品工业科技2017，Vol.38Issue(11)：55-60,6.DOI:10.13386/j.issn1002-0306.2017.11.002
响应面法优化多汁乳菇多糖提取工艺及抗氧化活性研究
Optimization of extraction process of polysaccharides from wild Lactarius volemus by response surface method and investigation of antioxidant activity
摘要
Abstract
In order to study the extraction process of wild Lactarius volemus polysaccharide (LVP)and its antioxidant activity,Central-Composite Design (CCD) of response surface design (RSD) was used to optimize the extract conditions of LVP based on single factor tests and O2 ·,· OH,DPPH,ABTS radicals scavenging activities were tested for antioxidant activity evaluation.The results showed that the optimal condition for extraction of LVP was as follows:temperature of 90 ℃,water bath time of 4 h,ratio of water to raw material of 33∶1 mL/g,corresponding yield of LVP was 2.18％,containing 55.40％ LVP,11.37％ protein and no starch.The IC50 of O2-·,· OH,ABTS and DPPH radicals scavenging activities of LVP were 868.16,280.00,342.06,167.65 μg/mL respectively.All in all,response surface design is reliable for the study of relationship between yield of LVP and temperature,water bath time,ratio of water to raw material,furthermore,LVP shows a higher antioxidant activity in radical scavenging experiments.关键词
多汁乳菇/多糖/提取/响应面/抗氧化活性Key words
Lactarius Volemus/polysaccharides/extraction/response surface design/antioxidant activity分类
轻工纺织引用本文复制引用
黄娟,黄燕燕,刘冬梅,陈素芹,潘伟才..响应面法优化多汁乳菇多糖提取工艺及抗氧化活性研究[J].食品工业科技,2017,38(11):55-60,6.基金项目
深圳市未来产业专项资金项目(CXZZ20140902155219302) （CXZZ20140902155219302）
国家自然科学基金项目(31101254) （31101254）
广东省科技计划项目(2014A020208019). （2014A020208019）
