食品工业科技2017，Vol.38Issue(11)：128-133,139,7.DOI:10.13386/j.issn1002-0306.2017.11.016
二维电泳技术对脉冲电场处理哈密瓜汁中酿酒酵母的蛋白质组学研究
Study on proteomics of Saccharomyces cerevisiae treated by pulsed electric field in Ha-mi melon juice using two dimensional electrophoresis technology
摘要
Abstract
2-dimensional gel electrophoresis (2-DE)technology was used to detect and analyze the protein of Saccharomyces cerevisiae in Ha-mi melon juice before and after PEF treatment.On the basis of the experiment results,the isoelectric point of protein spots was between 4.00 and 6.87,the molecular weight of protein spots was 14.58 ～ 109.83 kDa.The expression of proteins was up regulated in order to avoid the damage result in environment change,such as thioredoxin peroxidase and elongation factor 2.Additionally,because of field strength,genetic materials were affected,so the expression of nuclear chromatin protein and DNA repair protein was up regulated.The proteins like Cu-Zn cofactor superoxide dismutase and pyruvate phosphatese were down expressed.It revealed that cells viability decreased or cells were dead.The expression of cell survival and proliferation related proteins also changed significantly such as cystathionineβ-synthase,adenosine 5-single phosphate amide,atypical protein Ynr034w-A subunit and so on.The treatment of PEF affected the enzyme relevant to metabolize and genetic material in cells.It can provide the theoretical basis for the study of intensified sterilization of PEF.关键词
高压脉冲电场技术(PEF)/非热加工技术/二维电泳技术/酿酒酵母蛋白Key words
pulsed electric field (PEF)/non thermal processing technology/2-dimensional gel electrophoresis (2-DE)/Saccharomyces cerevisiae protein分类
轻工纺织引用本文复制引用
田一雄,赵伟,陈晓婵,杨瑞金..二维电泳技术对脉冲电场处理哈密瓜汁中酿酒酵母的蛋白质组学研究[J].食品工业科技,2017,38(11):128-133,139,7.基金项目
国家重点研发计划项目(2016YFD0400300) （2016YFD0400300）
国家自然基金科学(31522044) （31522044）
教育部新世纪优秀人才支持计划(NCET-13-0834) （NCET-13-0834）
中央高校基本科研业务费专项资金项目(JUSRP51406A). （JUSRP51406A）
