食品工业科技2017，Vol.38Issue(11)：140-143,4.DOI:10.13386/j.issn1002-0306.2017.11.018
乳酸菌氧化锆珠破壁方法的优化及适用性研究
Optimization of zirconia beads wall-breaking method for lactic acid bacteria and study on its effect of the applicability
摘要
Abstract
This study aims to optimize the zirconia beads wall-breaking method,screen out the better broken conditions for breaking lactic acid bacteria cell wall and improve the protein yield.The weight of bacteria,the grinding buffer and time were tested and selected as important aspects to optimize the wall-breaking method,and then,the breaking effect was analyzed and evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).Under optimized conditions,six different species of strains from lactic acid bacteria were tested for cell wall-breaking treatments with the zirconia beads method,after that,the breaking effects were analyzed and evaluated by SDS-PAGE.Results showed that,on condition that the ratio of bacteria,grinding buffer and zirconia beads (W/V/W)is 0.013 g∶300 μL∶1 g,grinding time of 15 min,the protein pattern with clear banding,high abundance;RIPA,PBS,PENP,GUS as the grinding buffer,the protein pattern with clear banding,high abundance,while 8 mol/L urea as the grinding buffer,the protein pattern with obscure banding,low abundance.After Six strains of lactic acid bacteria were broken through this method,the protein pattern with clear banding,high abundance.Therefore,zirconia beads method is a combined rapid and efficient method for breaking lactic acid bacteria cell wall.关键词
乳酸菌/氧化锆珠/优化/适用性Key words
lactic acid bacteria/zirconia beads/optimization/applicability分类
轻工纺织引用本文复制引用
潘荣荣,金宁一,王茂鹏,李昌,邸洋,荣凤君,杜寿文,朱羿龙,刘立明,朱国强..乳酸菌氧化锆珠破壁方法的优化及适用性研究[J].食品工业科技,2017,38(11):140-143,4.基金项目
国家自然科学基金(31472197) （31472197）
病原微生物生物安全国家重点实验室开放课题(SKLPBS1435). （SKLPBS1435）
