摘要
Abstract
Compared shake culture,two-stage culture process by combining shake culture with static cultureand solid-state fermentationof Ganoderma lucidum,analyzed the influence of different cultivation methods on total ganoderic acid,ganoderic acid Mk,ganoderic acid S,ganoderic acid T and ganoderic acid Me synthesis.Corn was used as the main medium to produce ganoderic acids,Plackett-Burman(PB)design was applied to screen potential key factors associated with total ganoderic acid content.Then,the steepest ascent experiment,central composite design and response surface methodology were applied to optimize the significant factors.The final results were as follows:39.0 g solid substrate (moisture content 38.0％) shake flask,1.08％ silkworm chrysalis powder,0.86％ sucrose,0.08％tri-ammonium citrate,0.22％ KH2PO4,0.13％ MgSO4,0.02％ VB1,culture temperature 29.5 ℃,and fermentation time 10 days.Under this condition,the total ganoderic acids content was 24.93 mg every gram of the ganoderma lucidum mycelium and corn powder,and ganoderic acid Mk,ganoderic acid S and ganoderic acid content reached 0.86,0.69 and 3.69 mg/g,respestively.Production of three ganodericacids by Ganoderma lucidum solid-state fermentation has good application prospect.关键词
灵芝菌丝体/固态发酵/Plackett-Burman(PB)设计法/响应面法Key words
Ganoderma lucidum mycelium/solid-state fermentation/Plackett-Burman design/response surface methodology分类
轻工纺织
