食品工业科技2017，Vol.38Issue(11)：258-265,8.DOI:10.13386/j.issn1002-0306.2017.11.041
响应面法优化苦水玫瑰精油提取后废弃物中总黄酮分离纯化工艺及抗氧化活性研究
Response surface optimization and purification technology and antioxidant activity of total flavonoids from Kushui rose oil distillation waste
摘要
Abstract
Objective:To investigate response surface optimization and purification technology and antioxidant activity of total flavonoids from Kushui rose oil distillation waste,and provide the basis for the recovcry of total flavonoids from Kushui rose oil distillation waste.Methods:Studying on D101 resin adsorption and desorption ability for flavonoids compounds by static experiment,then based on dynamic experiment of single factors,the optimum purification process parameters of Kushui rose oil distillation waste by response surface method.On this basis,with vitamin C (VC)as the control,study the scavenging ability of total flavonoids purified on ABTS,DPPH,and OH free radical.Results:The static adsorption capacity was 1.83 mg/g,the adsorption rate was 86.10％,the elution rate of 88.07％,which indicated that D101 resin had better adsorption and desorption ability for flavonoids compounds of Kushui rose oil distillation waste.And the optimum dynamic adsorption process parameter were the sample solution pH2.12,sample concentration 10.4 mg/L,sample flow rate 1.72 mL/min,the adsorption rate was 95.06％,elution with 60 mL absolute alcohol solution,the desorption rate reached 98.30％,the purity of total flavonoids was up to 5.651％.The IC50 value of total flavonoids purified on ABTS,DPPH,OH free radical were 0.03,0.1,8 μg/mL,the maximum scavenging rate were 98.96％,89.76％,65.45％,we can see that the total flavonoids from waste has better antioxidant activity.关键词
苦水玫瑰精油提取后废弃物/总黄酮/响应面/分离纯化/抗氧化活性Key words
Kushui rose oil distillation waste/total flavonoids/response surface/separation and purification/antioxidant activity分类
轻工纺织引用本文复制引用
陆秀云,王波,曹佳敏,李广,龚祥,周围..响应面法优化苦水玫瑰精油提取后废弃物中总黄酮分离纯化工艺及抗氧化活性研究[J].食品工业科技,2017,38(11):258-265,8.基金项目
甘肃省科技与支撑计划(农业类)(1604NKCA079). （农业类）
