食品工业科技2017，Vol.38Issue(11)：266-269,275,5.DOI:10.13386/j.issn1002-0306.2017.11.042
聚乙二醇-硫酸铵双水相体系萃取松花粉中总黄酮的工艺研究
Study on the extraction of total flavonoids from Pine Pollen in PEG400/(NH4) 2SO4 aqueous two-phase system
摘要
Abstract
An aqueous two-phase system of PEG400/(NH4)2SO4 was used to extract total flavonoids from Pine Pollen.The composition of the aqueous two-phase system was 26％ PEG400-20％ (NH4) 2SO4.Effects of the mass fraction of NaCl,pH and temperature of aqueous two-phase system on the partition ratio and extraction rate of total flavonoids from Pine Pollen were studied through the single factor experiments.The optimal extraction conditions were obtained by the orthogonal experimental design.The optimum conditions were as follows:the mass fraction of NaCl at 2％,pH at 4 and temperature at 40 ℃,and the extraction rate can reach up to 98.93％.The NaCl addition had the most effect on flavonoids partition.The aqueous two-phase extraction has the advantages of simple and couvenient procedure,moderate aqueous environment,shorter extraction time and higher extraction rate,which is effective for the separation and purification of total flavonoids.关键词
双水相体系/分配系数/萃取率/黄酮类化合物/松花粉Key words
Aqueous two-phase extraction/partition ratio/extraction rate/total flavonoids/Pine Pollen分类
轻工纺织引用本文复制引用
邵圣娟,卫静莉..聚乙二醇-硫酸铵双水相体系萃取松花粉中总黄酮的工艺研究[J].食品工业科技,2017,38(11):266-269,275,5.基金项目
太原工业学院院级科学基金(2014LY03). （2014LY03）
