食品工业科技2017，Vol.38Issue(11)：281-284,4.DOI:10.13386/j.issn1002-0306.2017.11.045
高纯度Nisin的制备及工业生产条件的优化
Preparation of high-purity Nisin and the optimization of industrial scale production conditions
陈艺强 1李光进 1金陈斌 1许峰 1叶艇 1洪超群1
作者信息
- 1. 浙江新银象生物工程有限公司,浙江台州317200
- 折叠
摘要
Abstract
In this study,a new method of preparation high-purity Nisin preparation was researched.The method adopted static adsorption and dynamic desorption of Nisin concentrated solution at a weak acidic ion exchange resin D113.The subsequent production process included salting out,suction filtration,drying and crushing to obtain high-purity Nisin.The results showed that the optimum condition was pH6,adsorption time 90 min,CDesorptionagent =1 mol/L,VHCl/VEtoHl =1.25,desorption rate 1 BV/h,C NaCl =23％ in salting out.The potency of Nisin attained through this procedure was 34000 IU/mg,total yield reached 67.45％.The method has bright prospect in industrial application.关键词
高纯度/乳酸链球菌素/离子交换Key words
high-purity/Nisin/ion exchange分类
轻工纺织引用本文复制引用
陈艺强,李光进,金陈斌,许峰,叶艇,洪超群..高纯度Nisin的制备及工业生产条件的优化[J].食品工业科技,2017,38(11):281-284,4.
