食品工业科技2017，Vol.38Issue(11)：352-355,4.DOI:10.13386/j.issn1002-0306.2017.11.060
西兰花茎叶多肽的酶法提取及其增强免疫力功能研究
Immunomodulatory effects of the enzymatic extract polypeptide from broccoli stems and leaves
摘要
Abstract
The polypeptide extract was gained by enzymatic method from broccoli stems and leaves and its immunomodulatory effects had been studied.The crude protein was extracted from broccoli stems and leaves,and then the broccoli polypeptides were prepared by enzymatic methods.The hydrolysate was isolated by the method of ultrafiltration and Sephadex G-15.The molecular mass of the oligopeptide was 310-2600 Da.The animal experiments were studied on their immunity function.Oligopeptide were consecutively orally given to mice with the doses of 100,50,25 mg/kg· bw(comparatively as 20,10,5 times of recommended human daily dosage) for 30 days.It was determinated for the humoral immune function,the cellular immune function and the mononuclear macrophage function in mice.Results showed that the high dose group (100 mg/kg)of broccoli peptide extracts had high conversion ability (0.225 + 0.040)and the ear weight were different (18.19 + 2.94) mg,it was significantly higher than that of control group (p ＜ 0.05).Broccoli polypeptide extracts have obvious effect on the normal mice carbon clearance function,carbon clearance indcx wcrc (0.126 + 0.052)and (0.136 + 0.022),high and medium dosc significantly (p ＜ 0.05)increased the nonspecific immune function of mice.Resuhs showed that the broccoli polypeptide extract could enhance immunity function,which could provide basic data and the scientific reference for the deep development and comprehensive utilization of broccoli.关键词
西兰花/多肽/提取/增强免疫力Key words
broccoli/polypeptide/extraction/enhancing immunity function分类
轻工纺织引用本文复制引用
党亚丽,么春艳,周亭屹,俞盈,李青青,吴庆其..西兰花茎叶多肽的酶法提取及其增强免疫力功能研究[J].食品工业科技,2017,38(11):352-355,4.基金项目
浙江省重大科技计划项目(2014C02023) （2014C02023）
浙江省医药卫生项目(201473497). （201473497）
