食品工业科技2017，Vol.38Issue(12)：21-24,38,5.DOI:10.13386/j.issn1002-0306.2017.12.004
HPLC测定半枝莲中野黄芩苷、黄芩素、木犀草素和芹菜素的含量
Determination of scutellarin, baicalein, luteolin and apigenin in Herba Scutellariae barbatae by HPLC
摘要
Abstract
To establish HPLC methods for simultaneous determination of scutellarin,baicalein,luteolin and apigenin in Herba Scutellariae barbatae.The chromatographic conditions were as follows-A Diamonsil-C18 (250 mm × 4.6 mm,5 μm) was choosed as the chromatographic column,the mobile phase was the mixture of acetonitril(A) and 1％ glacial acetic acid in water(B) with gradient elution,the detection wavelength at 335 nm,the flow velocity of 1.0 mL/min,the column temperature at 30 ℃C.The linear relationship between content and peak area was obtained during the range of 2.0～200 μg/mL for scutellarin,0.5～50 μg/mL for baicalein,0.5～50 μg/mL for luteolin and 0.5～50 μg/mL for apigenin.RSD' s founds of the peak area of scutellarin,baicalein,luteolin and apigenin for the precision test,the stability test and the repeatahility test were less than 2.0％.The recoveries,average recovery and RSDs of scutellarin,baicalein,luteolin and apigenin obtained were 97.73％～ 101.7％,99.72％,1.36％ (n =9),97.78％ ～ 102.8％,99.53％,1.99％ (n =9),97.40％ ～ 102.4％,99.39％,1.84％ (n =9) and 97.80％ ～ 102.0％,99.24％,1.49％ (n =9).By using the established HPLC methods,12 samples of Herba Scutellariae barbate in different districts were analyzed.The results showed that the contents of scutellarin,baicalein,luteolin and apigenin were 2.41～7.08,0.26～0.56,0.31～0.90 mg/g and 0.28 ～0.95 mg/g,respectively.The method could be applied to simultaneous quantitativc assay of scutellarin,baicalein,luteolin and apigenin in Herba Scutellariae barbatae with good specificity,reproducibility and stability.The sampling recovery test is in compliance with the requirement.关键词
半枝莲/野黄芩苷/黄芩素/木犀草素/芹菜素/高效液相色谱法Key words
Herba Scutellariae barbatae/scutellarin/baicalein/luteolin/apigenin/HPLC分类
轻工纺织引用本文复制引用
周溦,林敬明,王索丽,林海桢..HPLC测定半枝莲中野黄芩苷、黄芩素、木犀草素和芹菜素的含量[J].食品工业科技,2017,38(12):21-24,38,5.基金项目
广东省自然科学基金(S2013010014796). （S2013010014796）
