食品工业科技2017，Vol.38Issue(12)：34-38,5.DOI:10.13386/j.issn1002-0306.2017.12.007
乳酸菌破壁方法的比较研究
Comparision research on the cell wall-breaking methods of lactic acid bacteria
摘要
Abstract
This study aims to explore a rapid,efficient and economical method for breaking lactic acid bacteria cell wall.Five different methods including glycine treatment,repeated freezing and thawing,lysozyme digestion,zirconia beads disruption,ultrasonic treatment and three composite wall-breaking methods were used to treat Lactobacillus.plantarum 1.557 cells,respectively.And gram staining and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)were used as evaluation method to analyze the difference of cell wall-breaking effects and time costs.In addition,the activity of protein extracted by zirconia beads method was tested by theβ3-glucuronidase(GUS) assays.The quality of RNA extracted by zirconia beads method was tested by agarose gel electrophoresis.Results showed that zirconia beads method was better than other methods,the GUS protein extracted by this method had the activity of protein.The OD260/OD280 ratio of RNA extracted by this method was between 1.8 and 2.1,which was in accordance with the standard ratio,and the purity was high and the degradation degree was low.Therefore,the zirconia beads method was a fast,efficient and economical method for rapid extraction of lactic acid bacteria bacterial protein and total RNA.关键词
乳酸菌/破壁方法/氧化锆珠/蛋白提取/RNA提取Key words
lactic acid bacteria/cell wall-breaking method/zirconia beads/protein extraction/RNA extraction分类
轻工纺织引用本文复制引用
潘荣荣,金宁一,王茂鹏,李昌,邸洋,荣凤君,杜寿文,朱羿龙,刘立明,朱国强..乳酸菌破壁方法的比较研究[J].食品工业科技,2017,38(12):34-38,5.基金项目
国家自然科学基金(31472197) （31472197）
病原微生物生物安全国家重点实验室开放课题(SKLPBS1435). （SKLPBS1435）
