中国食品学报2017，Vol.17Issue(6)：24-32,9.DOI:10.16429/j.1009-7848.2017.06.003
鳕鱼皮胶原蛋白肽对小鼠慢性肝损伤的保护机制
Propection Mechanism of Cod Skin Collagen Peptide on Chronic Liver Injury in Mice
摘要
Abstract
Objective:To investigate the protective effects of cod skin collagen peptide,which will provide evidences in developing function food.Methods:Chronic liver injury models were set by intraperitoneal injection CCl4 in mice.The activities of ALT,AST in serum and SOD,the contens of liver MDA were determined.Liver tissue was stained with HE for observing the effect of CSCP on mice liver histopathology.Expression of Bax,Bcl-2,Cleavage Caspase-3,Caspase-9,Cytosolic Cyt c,TNF-α,JNK,P-JNK protein were measured by western blot analysis.Reasults:Compared with model group,the groups high CSCP (400 mg/kg),ALT,AST activities in serum,MDA conten in liver were declined significantly,SOD activities significantly increased;the liver histology was improved;Expression levels of Bcl-2 improved,Bax,Cleavage Caspase-3,Caspase-9,Cytosolic Cyt c,TNF-α,JNK,P-JNK were all improved in the CSCP group.Conclusion:The cod skin collagen peptide enhanced the antioxidant activity,inhibited the oxidative stress,effectively protect the chronic liver injury by CCl4 in mice.关键词
鳕鱼皮/胶原蛋白肽/慢性肝损伤/氧化应激Key words
cod skin/collagen peptide/chronic liver injury/oxidative stress引用本文复制引用
刘晨晨,丁国芳,贾盈露,王加斌,赵玉勤,杨最素..鳕鱼皮胶原蛋白肽对小鼠慢性肝损伤的保护机制[J].中国食品学报,2017,17(6):24-32,9.基金项目
浙江省科技厅重大专项(2013C03036,2011C02003) （2013C03036,2011C02003）
舟山市科技计划项目(2012C21013) （2012C21013）
