中国食品学报2017，Vol.17Issue(6)：40-46,7.DOI:10.16429/j.1009-7848.2017.06.005
虾青素对脐静脉内皮细胞的抗氧化作用
Antioxidative Effects of Astaxanthin on Human Umbilical Vein Endothelial Cells
摘要
Abstract
Objective:To explore the antioxidant effects of astaxanthin on human umbilical vein endothelial cells (HUVEC).Methods:HUVEC cells were divided into normal control group,model group (2 mmol/L H2O2),and astaxanthin+H2O2 (pretreated with 0.1,1,10 μmol/L astaxanthin for 48 h and then cultured with 2 mmol/L H2O2 for 1 h).The cell survival was measured by MTT method,the level of reactive oxygen species was determined by DCFH-DA,the changes of mitochondrial membrane potential and apoptosis ratio were detected by JC-1 method,and Annexin V-FITC and DAPI methods.The expression of Caspase-3 and p53 were measured by western blot.Results:Compared with control group,2 mmol/L H2O2 obviously caused the necrosis and apoptosis of HUVEC,while astaxanthin could reduce this damage,which was presented by the significantly decreased cell necrosis ratio,increased MMP and down-regulated Caspase-3 and p53 expression.Conclusion:Astaxanthin inhibited oxidative stress induced cell death of H2O2,and its mechanisms may be related to protecting the mitochondrial function.关键词
虾青素/氧化损伤/脐静脉内皮细胞/细胞凋亡/线粒体膜电位Key words
astaxanthin/oxidative stress/HUVEC cells/cell apoptosis/mitochondrial membrane potential引用本文复制引用
牛婷婷,王峰,吴玮,陈海敏,高鑫..虾青素对脐静脉内皮细胞的抗氧化作用[J].中国食品学报,2017,17(6):40-46,7.基金项目
国家自然科学基金项目(81370532) （81370532）
浙江省医药卫生科技计划项目(2013KYB238) （2013KYB238）
宁波市自然科学基金项目(2013A610220) （2013A610220）
