中国食品学报2017，Vol.17Issue(7)：34-40,7.DOI:10.16429/j.1009-7848.2017.07.005
竹茹多糖的化学结构及体外免疫活性研究
Structure and in vitro Immunomodulatory Activity of Polysaccharides from Bamboo (Phyllostachys pubescens Mazel) Shavings
黄菊青 1齐睿婷 1张英1
作者信息
- 1. 浙江大学生物系统工程与食品科学学院浙江省农产品加工技术研究重点实验室浙江省食品加工技术与装备工程中心 杭州310058
- 折叠
摘要
Abstract
Objective:to investigate the structure and in vitro immunomodulatory activity of polysaccharides from bamboo shavings (BSP).Methods:the structure was analyzed by the methods of methylation and nuclear magnetic resonance spectroscopy;the in vitro immunomodulatory activity was evaluated by the assays of mouse splenic lymphocyte proliferation,the phagocytosis of neutral red and NO secretion of RAW264.7 cell.Results:BSP was O-acetyl-(4-O-methylglucurono)-arabinoxylans consisting of a linear (l→4)-β-D-xylopyranosyl backbone decorated with branches at O-2 of 4-O-methylglucuronic acid units and acetyl groups or O-3 of α-L-arabinofuranosyl and acetyl groups.The structural element could be expressed as below:1,4-β-D-[2-O-Ac][3-O-Ac][4-O-Me-D-GlcpA-(1→2)][α-L-Araf-(1→3)]-xylp;BSP could evidently strengthen concanavalin A or lipopolysaccharide-induced splenic lymphocyte proliferation at 10-100μg/mL;BSP could significantly enhance the phagocytosis activity and NO production of RAW264.7 cell at 10-1 000 μg/mL in a dose-dependent manner.Conclusion:BSP exhibits excellent immunostimulatory activity in vitro,and can be considered as an ideal source of bio-active polysaccharides due to the abundance of its raw material.关键词
竹茹/多糖/化学结构/体外免疫活性Key words
bamboo shavings/polysaccharides/structure/in vitro immunomodulatory activity引用本文复制引用
黄菊青,齐睿婷,张英..竹茹多糖的化学结构及体外免疫活性研究[J].中国食品学报,2017,17(7):34-40,7.
