中国食品学报2017，Vol.17Issue(7)：199-207,9.DOI:10.16429/j.1009-7848.2017.07.025
酒酒球菌β-葡萄糖苷酶基因克隆及生物信息学分析
Cloning and Bioinformatic Analysis of the β-glucosidase Gene in Oenococcus oeni
摘要
Abstract
[Objective]The aim of the work is to reveal the construction features ofβ3-glucosidase gene (bgl) in Oenococcus oeni in the molecular level and to provide a theoretical direction for further exploring the β-glucosides metabolism in O.oeni cells.[Methods] The target gene was cloned by PCR from the genomic DNA of four O.oeni strains (SD-ld,SD-2gf,31-DH and NM-2c),and the encoded productions were analyzed by bioinformatics technology.[Results] The results showed that the bgl gene has a completely open reading frame,being 2215 bp and encoding 737 amino acids.The initiation codon is TTG and the termination codon is TAA.Bioinformatic analysis indicated that the encoded protein was of weak hydrophobicity,no signal peptide and transmembrane helice,stabilization and heat-resistance,belonging to the superfamily of Glyco_hydro_3.[Conclusion] The encoded productions of bgl gene were a protein of 81625.3 u and showed BGL activity.It was considered to be non-secretory cytoplasmic protein and located in cytoplasm.关键词
酒酒球菌/β-葡萄糖苷酶/基因克隆/生物信息学分析Key words
Oenococcus oeni/β-glucosidase/gene cloning/bioinformatics technology引用本文复制引用
杨世玲,刘叶,薛楚然,王玲,何玲,刘树文..酒酒球菌β-葡萄糖苷酶基因克隆及生物信息学分析[J].中国食品学报,2017,17(7):199-207,9.基金项目
国家重点研发计划项目(2016YFD0400504) （2016YFD0400504）
国家现代农业产业技术体系建设专项(nycytx-30-ch-03) （nycytx-30-ch-03）
