中国食品学报2017，Vol.17Issue(7)：255-262,8.DOI:10.16429/j.1009-7848.2017.07.032
离子色谱-电感耦合等离子体质谱联用测定茶叶中4种砷形态
Determination of Four Arsenic Species in Tea by Ion Chromatography-inductively Coupled Plasma-mass Spectrometry
摘要
Abstract
A method was developed for the simultaneous determination of arsenic acid [As(V)],arsenious acid [As (Ⅲ)],monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in tea by ion chromatography-inductively coupled plasma-mass spectrometry (IC-ICP-MS).Three kinds of extract solutions were compared by extraction efficiency and the separation parameters were optimized.The extraction regent was 0.2 mol ·L-1 nitric acid with heat-assistant condition for 2 h at 100 ℃.Then the 4 arsenic species were separated by an anion exchange column (Dionex Ion Pac As7,250 mm×2 mm) and detected by ICP-MS.The recoveries of the 4 arsenic species spiked in tea at three levels ranged from 90.9％ to 125.0％ with the relative standard deviations (RSDs,n=4) less than 5.7％.Detction limits of As (V),As (Ⅱ-I),MMA and DMA were 0.1,0.2,0.3 and 0.3 μ,g·L-1,corresponding to 2.5,5.0,7.5 and 7.5 μg·kg-1 in tea samples,respectively.This method is applied to investigate the background content of the 4 arsenic species in 4 kind of commercial tea samples,and the content of As (V),As (Ⅲ),MMA and DMA were 0-545.1,0-147.0,0-95.2 and 0-86.6μg·kg-1.The contents of inorganic arsenic was higher than that organic arsenic significantly (P＜0.05),and the average contents were As (V),As (Ⅲ),MMA and DMA in order.The result shows that this method is simple and precise for the determination and risk assessment of four arsenic species in tea.关键词
砷形态/茶叶/离子色谱/电感耦合等离子体质谱/硝酸消解Key words
arsenic speciation/tea/ion chromatography (IC)/inductively coupled plasma mass spectrometry (ICP-MS)/nitric acid digestion引用本文复制引用
章剑扬,王国庆,马桂岑,金寿珍,鲁成银,刘新..离子色谱-电感耦合等离子体质谱联用测定茶叶中4种砷形态[J].中国食品学报,2017,17(7):255-262,8.基金项目
现代农业产业技术体系建设专项(nycytx-26) （nycytx-26）
中国农业科学院创新团队(CAAS-ASTIP-2014-TRICAAS) （CAAS-ASTIP-2014-TRICAAS）
浙江省自然科学基金(Q15C16002) （Q15C16002）
