葛根素促进体外培养大鼠颅骨成骨细胞增殖和分化过程中对cAMP/PKA信号通路的影响OACSTPCD
Effects of Puerarin in Promoting Proliferation and Differentiation of Rats' Osteoblast by Culture in Vitro on cAMP/PKA signal Pathway
目的 研究葛根素对体外培养大鼠颅骨成骨细胞(rats osteoblast,ROBs)增殖和分化及cAMP/PKA信号通路的影响.方法 体外分离培养ROBs,观察不同浓度(1×10-4～1 ×10-8 mol/L)葛根素对ROBs增殖和碱性磷酸酶(alkaline phosphatase,ALP)活性的影响;分析葛根素对骨形态发生蛋白(Bone morphogenetic protein,BMP-2)、Ⅰ型胶原(Col Ⅰ)、成骨特异性转录因子-…查看全部>>
Objective To investigate effects of Puerarin on promoting proliferation and differentiation of rats'osteoblasts (ROBs) by culture in vitro on cAMP/PKA signal pathway.Methods ROBs were isolated and cultured in vitro,and effects of different concentrations Puerarin (1 × 10-4 mol · L-1-× 10-8 mol · L-1) on proliferation and alkaline phosphatase (ALP) activity of ROBs were observed.Effects of Puerarin on bone morphogenetic protein-2 (BPM-2),type Ⅰ collagen (Col …查看全部>>
邓银栓;樊宇;惠升明;杨小杰;董成辉
730050兰州,兰州军区兰州总医院运动医学科730050兰州,兰州军区机关门诊部730050兰州,68202部队医院730050兰州,兰州军区兰州总医院运动医学科730050兰州,兰州军区兰州总医院运动医学科
医药卫生
葛根素成骨细胞颅骨细胞增殖细胞分化大鼠,Wistar
PuerarinOsteoblastSkullCell proliferationCell differentiationRats, Wistar
《解放军医药杂志》 2017 (7)
5-9,5
军区医药卫生课题(CLZ14JA05)
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