食品科学2017，Vol.38Issue(15)：1-6,6.DOI:10.7506/spkx1002-6630-201715001
肌原纤维蛋白磷酸化对其被μ-钙蛋白酶降解的影响
Effect of Phosphorylation on the Degradation of Myofibrillar Proteins by μ-Calpain
摘要
Abstract
Objective:The objective of this study was to investigate the effect of phosphorylation on the degradation of myofibrillar proteins by μ-calpain.Methods:Protein kinase A (PKA) and alkaline phosphatase (AP) were used to catalyze the phosphorylation and dephosphorylation of myofibrillar proteins of mutton longissimus dorsi muscle,followed by hydrolysis by μ-calpain.The levels of protein phosphorylation and degradation were measured by SDS-PAGE,Pro-Q Diamond fluorescent staining,and Western blotting,respectively.Results:The phosphorylation level of myofibrillar proteins in the PKA group was significantly higher than that of the control group (P ＜ 0.05),while the phosphorylation level of myofibrillar proteins in the AP group was significantly lower than that of the control group (P ＜ 0.05).The autolysis and activity of μ-calpain were suppressed by PKA treatment,thereby leading to prolonged hydrolysis of myofibrillar proteins by μ-calpain.Phosphorylation enhanced the degradation of myosin heavy chain and troponin T,while dephosphorylation enhanced the degradation of actin and desmin.Conclusion:Phosphorylation affected both myofibrillar proteins and μ-calpain activity and therefore the degradation of myofibrillar proteins by μ-calpain.关键词
磷酸化/肌原纤维蛋白/μ-钙蛋白酶/降解/蛋白激酶A/碱性磷酸酶Key words
phosphorylation/myofibrillar proteins/μ-calpain/degradation/protein kinase A/alkaline phosphatase分类
轻工纺织引用本文复制引用
李铮,李欣,杜曼婷,李蒙,张德权..肌原纤维蛋白磷酸化对其被μ-钙蛋白酶降解的影响[J].食品科学,2017,38(15):1-6,6.基金项目
国家自然科学基金面上项目(31471604) （31471604）
中国博士后科学基金面上项目(2015M571176) （2015M571176）
国家现代农业(肉羊)产业技术体系建设专项(CARS-39) （肉羊）
