食品科学2017，Vol.38Issue(15)：43-50,8.DOI:10.7506/spkx1002-6630-201715008
Fe3+修饰磁性纳米粒子的制备与表征及对卵黄高磷蛋白的吸附作用
Synthesis and Characterization of Surface-Modified Magnetic Nanoparticles for Phosvitin Adsorption
摘要
Abstract
Functional magnetic nanoparticles Fe3O4-CS@Fe3+ (CMNP@Fe3+ for short) were prepared by chemical co-precipitation and functional modification with sodium chondroitin sulfate and ferric ion,and their surface morphology was analyzed.CMNP@Fe3+ showed a spherical shape with a small diameter of about 20 nm,and they could be well dispersed in aqueous solution as proved by transmission electron microscope.It was found that the nanoparticles had superparamagnetic properties as indicated by the hysteresis loop.Fourier transform infrared spectrum revealed that sodium chondroitin sulfate and Fe3+coated the surface of Fe3O4 successfully.This study established a new method to extract phosvitin from hen egg yolk by taking advantage of the strong binding of Fe3+ on the surface of magnetic nanoparticles to phosvitin,and we also investigated the factors influencing the adsorption process.The magnetic nanoparticles had maximum adsorption capacity under the following conditions:solution pH 4.0,initial substrate concentration 10 mg/mL,and adsorption time 180 min.The adsorption kinetics of CMNP@Fe3+ for phosvitin was better described by a pseudo second-order kinetic model,and the theoretical adsorption capacity at the equilibrium state computed from the model was 625.00 mg/g.The adsorption data were fit to well the Freundlich isotherm model.This research may provide a reference for protein separation from eggs using magnetic particles.关键词
卵黄高磷蛋白/磁性纳米粒子/磁性分离/硫酸软骨素/铁离子Key words
phosvitin/magnetic nanopaticles/magnetic separation/sodium chondroitin sulfate/ferric ion分类
轻工纺织引用本文复制引用
陈婵,黄茜,李珊珊,马美湖..Fe3+修饰磁性纳米粒子的制备与表征及对卵黄高磷蛋白的吸附作用[J].食品科学,2017,38(15):43-50,8.基金项目
国家自然科学基金面上项目(31471602) （31471602）
公益性行业(农业)科研专项(201303084) （农业）
