食品科学2017，Vol.38Issue(15)：140-146,7.DOI:10.7506/spkx1002-6630-201715023
鸡肉源沙门氏菌血清型、药敏性及部分耐药基因研究
Serotyping, Antibiotic Susceptibility and Some Genes Associated with Quinolones and Fluoroquinolines Resistance of Chicken-Borne Salmonella
摘要
Abstract
Objective:In this study,the serotyping,antibiotic susceptibility and some genes associated with quinolone and fluoroquinolones resistance of 432 chicken-borne Salmonella isolates in and around Yangling,Shaanxi province were studied for better understanding the epidemiological characteristics of Salmonella serovars.Methods:Salmonella serovars were determined using slide agglutination method.Antibiotic susceptibility was tested by the agar dilution method recommended by the Clinical and Laboratory Standards Institute (CLSI).The resistant genes of qnrA,qnrB,qnrS and aac(6')-Ib-cr,commonly carried by plasmid and associated with resistance to quinolone and fluoroquinolones,were identified using PCR and DNA sequencing.Results:Totally 37 serotypes were identified among 396 Salmonella isolates,and the other 36 isolates were unidentified.The most prevalent serotype was Salmonella typhimurium (17.93％),followed by S.thompson (12.88％),S.essen (8.84％) and S.infantis (6.57％).Among 432 Salmonellae isolates,antibiotic resistance was commonly found to sulfafurazole (89.81％),and approximately 50％ of the isolates were resistant to sulfamethoxazole,naladixic acid,tetracycline,ampicillin,chloramphenicol,and amoxicillin/clavulanate potassium,aac(6')-Ib-cr (16.67％) was most frequently detected,and the detection rates of qnrB,qnrS and qnrA were 10.19％,6.71％ and 1.62％,respectively.The distribution of these four genes differed in different serotypes of isolates.More qnr and aac(6')-Ib-cr genes were detected in the isolates found to be more resistant to both fluoroquinolones and aminoglycosides.Conclusion:The serotype of Salmonella isolates from retail chicken in Yangling and its surrounding areas was diverse,and qnr and aac(6')-Ib-cr genes were commonly found in these isolates that were resistant to many antibiotics.关键词
沙门氏菌/血清型/(氟)喹诺酮类抗生素/耐药基因Key words
Salmonella/serotype/fluoroquinolones/antibiotic resistant genes分类
轻工纺织引用本文复制引用
王嘉炜,肖英平,杨华,曹晨阳,杨保伟..鸡肉源沙门氏菌血清型、药敏性及部分耐药基因研究[J].食品科学,2017,38(15):140-146,7.基金项目
浙江省农业科学院“农产品质量安全”中美国际合作项目 （）
国家自然科学基金面上项目(31171682) （31171682）
