食品科学2017，Vol.38Issue(15)：183-188,6.DOI:10.7506/spkx1002-6630-201715030
亲和毛细管电泳法和荧光猝灭法分析α-乳白蛋白与离子液体相互作用
Interactions of α-Lactalbumin with Ionic Liquids Analyzed by Affinity Capillary Electrophoresis and Fluorescence Quenching Method
摘要
Abstract
An amino acid ionic liquid (AAIL),1-ethyl-3-methylimidazolium 1-aminopropane-1,3-dicarboxylic acid salt ([C2mim][Glu]),was synthesized by the neutralization method from 1-ethyl-3-methylimidazolium bromide ([C2mim]Br).The binding behavior of α-lactalbumin (α-LAB) with [C2mim][Glu] and [C2mim]Br was evaluated by affinity capillary electrophoresis and fluorescence quenching method.The binding constants of both α-LAB-[C2mim][Glu] and α-LAB-[C2mim]Br were in the order of magnitude of 106 L/mol,with the former being higher than the latter,and the binding ratios were approximately 1∶1.This research may provide some references for further studies aimed to understand the mechanism behind α-LAB-ionic liquid interaction and develop an efficient and environment-friendly extraction system for α-LAB.关键词
α-乳白蛋白/氨基酸离子液体/亲和毛细管电泳/结合常数/荧光猝灭法Key words
α-lactalbumin (α-LAB)/amino acid ionic liquids (AAIL)/affinity capillary electrophoresis (ACE)/binding constant/fluorescence quenching method分类
化学化工引用本文复制引用
贾娜尔·吐尔逊,王晓娅,谭瑞康,李德强..亲和毛细管电泳法和荧光猝灭法分析α-乳白蛋白与离子液体相互作用[J].食品科学,2017,38(15):183-188,6.基金项目
新疆农业大学校前期资助课题(XJAU201204) （XJAU201204）
