食品科学2017，Vol.38Issue(15)：208-214,7.DOI:10.7506/spkx1002-6630-201715034
异牛肝菌素通过阻断SM22α泛素化降解抑制血管平滑肌细胞表型转化
Iso-Suillin Inhibits VSMC Phenotypic Modulation via Repressing Ubiquitination-Mediated SM22α Degradation
摘要
Abstract
Iso-suillin,a phenolic compound isolated from Suillus flavus,has significant anti-tumor activity.However,the role of iso-suillin in cardiovascular system remains unclear.Herein,primary vascular smooth muscle cells (VSMCs) were pretreated with iso-suillin for 24 or 48 h,and then stimulated by platelet-derived growth factor (PDGF)-BB.The cell proliferation was evaluated by cell counting.VSMC cell cycle distribution was analyzed by flow cytometry.In addition,Western blot was used to examine protein expression,and immuneprecipitation analysis was used to identify the phosphorylation and ubiquitination of smooth muscle 22 alpha (SM22α).The results showed that iso-suillin inhibited PDGF-BB-induced VSMC proliferation.Iso-suillin increased the expression of SM22α (a marker of cell differentiation),and reduced the level of PCNA.Furthermore,iso-suillin decreased the levels of phosphorylated and ubiquitinated SM22α upon PDGF-BB treatment,which may be associated with iso-suillin-decreased protein kinase C (PKC) δ activity.Collectively,these data demonstrated that iso-suillin prevented VSMC phenotype transformation through decreasing PKCδ-mediated SM22α degradation via the ubiquitin-proteasome pathway in VSMCs.关键词
异牛肝菌素/血管平滑肌细胞/表型转化/平滑肌22α (SM22α)Key words
iso-suillin/vascular smooth muscle cells/phenotypic modulation/smooth muscle 22α (SM22α)分类
医药卫生引用本文复制引用
吕品,范素芳,宋楠,王璐琪,孙勇,赵俊霞,张岩..异牛肝菌素通过阻断SM22α泛素化降解抑制血管平滑肌细胞表型转化[J].食品科学,2017,38(15):208-214,7.基金项目
“十三五”国家重点研发计划重点专项(2016YFF0202300) （2016YFF0202300）
科技部质检行业公益专项(201510208-08) （201510208-08）
国家自然科学基金青年科学基金项目(81300225) （81300225）
河北省自然科学基金资助项目(H2015206467) （H2015206467）
河北省高等学校优秀青年基金项目(YQ2013018) （YQ2013018）
