食品科学2017，Vol.38Issue(16)：10-16,7.DOI:10.7506/spkx1002-6630-201716002
变形假单胞菌2-酮基葡萄糖酸激酶基因的克隆、表达及生物信息学分析
Cloning, Expression and Bioinformatic Analysis of 2-Ketogluconate Kinase Gene from Pseudomonas plecoglossicida
摘要
Abstract
The full-length sequence of 2-ketegluconate kinase gene (kguK) from was cloned from an industrial 2KGA producer of Pseudomonas plecoglossicida JUIM01 and expressed in the recombinant strain Escherichia coli BL21(DE3)/ pET-28a(+)-kguK with isopropyl β-D-1-thiogalactopyranoside induction.The specific fusion protein KguK had a molecular weight of about 36.0 ku,which was confirmed by Western-Blot.The bioinformatics analysis showed that KguK in P.plecoglossicida was a hydrophilic protein with 305 amino acid residues.It was located in the cytoplasm,having a conserved domain similar to that of the pfkB family.The predicted secondary structure contained 35.73％ of α-helixes,12.79％ of extended strand and 51.48％ of random coil.关键词
变形假单胞菌/2-酮基葡萄糖酸激酶/kguK基因/克隆/表达/生物信息学Key words
Pseudomonas plecoglossicida/2-ketegluconate kinase (KguK)/kguK gene/cloning/expression/bioinformatics分类
生物科学引用本文复制引用
何小用,王大明,孙文敬,崔凤杰,钱静亚,齐向辉,余泗莲..变形假单胞菌2-酮基葡萄糖酸激酶基因的克隆、表达及生物信息学分析[J].食品科学,2017,38(16):10-16,7.基金项目
国家自然科学基金面上项目(31571885) （31571885）
国家高技术研究发展计划(863计划)项目(2012AA022103) （863计划）
江西省科技计划项目(赣知发[2015]64号) （赣知发[2015]64号）
江西省创新团队建设计划项目(20142BCB24024) （20142BCB24024）
江西省科技平台建设计划项目(2010DTZ01900) （2010DTZ01900）
德兴市科技计划项目(德科发[2015]44号) （德科发[2015]44号）
