食品科学2017，Vol.38Issue(16)：17-22,6.DOI:10.7506/spkx1002-6630-201716003
单核增生性李斯特菌hly缺失菌株的构建及生物特性的初步鉴定
Construction and Biological Characterization of hly Gene Knockout Listeria monocytogenes Mutant Strains
摘要
Abstract
This study aimed to elucidate the effect of hly gene on the virulence ofListeria monocytogenes.We constructed a hly gene knockout mutant strain EGD-e△hly from wild-type L.monocytogenes EGD-e by using a shuttle vector through homologous recombination.Biological characterization showed that there was no difference between the growth status of the mutant and parent strains.However,the mutant strain lost hemolytic activity,the cell invasion ability was decreased and there was no toxicity observed in animals receiving intraperitoneal injection at a concentration of 107 cells/mL.At the transcriptional level,the knockout of hly gene down-regulated the expression levels of virulence genes inlC and prfA by 81％ and 76％ respectively and up-regulated the expression levels of actA and plcB genes by 2.7 and 1.8 times,respectively.This paper may provide a basis for the study of the pathogenic mechanism of Lm.关键词
单核增生性李斯特菌/hly基因/同源重组/生物特性Key words
Listeria monocytogenes/hly gene/homologous recombination/biological characteristics分类
轻工纺织引用本文复制引用
谢曼曼,刘武康,丁承超,董庆利,陈国薇,曾海娟,郭亮,刘箐..单核增生性李斯特菌hly缺失菌株的构建及生物特性的初步鉴定[J].食品科学,2017,38(16):17-22,6.基金项目
国家自然科学基金面上项目(31371776) （31371776）
上海理工大学微创励志创新基金项目(YS30809101) （YS30809101）
上海理工大学研究生创新基金项目 （）
