食品科学2017，Vol.38Issue(16)：23-28,6.DOI:10.7506/spkx1002-6630-201716004
酒曲中产凝乳酶微生物菌株的分离筛选及鉴定
Isolation and Identification of Microbial Strains Producing Rennet from Jiuqu, a Traditional Chinese Fermentation Starter
摘要
Abstract
Eleven bacterial strains and two fungal strains were isolated from Jiuqu,a traditional Chinese fermentation starter for rice wine.Out of these,one bacterial strain,designated LB-51,capable of producing rennet was screened by the casein plate method and the Arima method.The strain was identified as Bacillus amyloliquefaciens by its morphological characteristics,physiological and biochemical tests (API 50CHB system) and 16S rDNA sequence analysis and species specific gene analysis and named as B.amyloliquefaciens GSBa-1.The rennet and proteolytic activities were (431.53 ± 15.89) SU/mL and (5.05 ± 0.59) U/mL,respectively,after 72 h shaking culture (120 r/min) at 30 ℃ for 72 h in liquid LB medium.The enzyme exhibited high milk-clotting activity and low protein hydrolysis activity.The milk-clotting activity was 1.54 × 105 SU/g.B.amyloliquefaciens GSBa-1,an efficient producer and a safe source of rennet activity,is worth further research and development as a candidate strain for industrial production of rennet.关键词
酒曲/凝乳酶/分离鉴定/解淀粉芽孢杆菌Key words
Jiuqu/rennet/isolation and identification/Bacillus amyloliquefaciens分类
轻工纺织引用本文复制引用
腾军伟,赵笑,杨亚威,张健,赵爱梅,姜云芸,李柳,郑喆,杨贞耐..酒曲中产凝乳酶微生物菌株的分离筛选及鉴定[J].食品科学,2017,38(16):23-28,6.基金项目
国家自然科学基金面上项目(31371804) （31371804）
北京市百千万人才工程资助项目(B类) （B类）
国家自然科学基金青年科学基金项目(31601488) （31601488）
2016年北京工商大学研究生科研能力提升计划项目 （）
公益性行业(农业)科研专项(201303085) （农业）
