食品科学2017，Vol.38Issue(16)：29-35,7.DOI:10.7506/spkx1002-6630-201716005
泡盛曲霉液体发酵产β-1,3-1,4-葡聚糖酶的条件优化
Optimization of Liquid-State Fermentation Conditions for β-1,3-1,4-Glucanase (Lichenase) Production by Aspergillus awamori
摘要
Abstract
The liquid-state fermentation conditions for β-1,3-1,4-glucanase production by Aspergillus awamori CAU33,a fungal strain isolated from soil sample,were optimized using a combination of one-factor-at-a-time method and response surface methodology.The highest β-1,3-1,4-glucanase activity of 8 447 U/mL was achieved after 6 days of culture at 35 ℃ in a medium consisting of corncob 55 g/L,soybean peptone 25 g/L,and Triton X-114 23 g/L at an initial pH of 4.5,about 17.6 times higher than before the optimization.关键词
β-1,3-1,4-葡聚糖酶/液体发酵/优化/泡盛曲霉Key words
β-1,3-1,4-glucanase/liquid-state fermentation/optimization/Aspergillus awamori分类
轻工纺织引用本文复制引用
刘二伟,杨绍青,闫巧娟,江正强..泡盛曲霉液体发酵产β-1,3-1,4-葡聚糖酶的条件优化[J].食品科学,2017,38(16):29-35,7.基金项目
国家自然科学基金面上项目(31471688) （31471688）
