食品科学2017，Vol.38Issue(16)：43-50,8.DOI:10.7506/spkx1002-6630-201716007
内蒙古地区绿豆品种遗传多样性SSR分析及DNA指纹图谱构建
Genetic Diversity Analysis and DNA Fingerprint Construction Based on Fluorescent Labeled SSR Markers for Mungbean Varieties (Vigna radiate L.) from Inner Mongolia
摘要
Abstract
Objective:The aim of this study was to analyze the genetic diversity of mungbean varieties from Inner Mongolia region and to establish a simple sequence repeat (SSR) fingerprinting database for providing scientific evidence to explore the genetic background of mungbean germplasms and authenticate mungbean varieties.Methods:A total of 92 mungbean varieties from Inner Mongolia area were taken to establish their genetic diversity and DNA fingerprint maps by using 10 pairs of SSR primers.Results:A total of 58 alleles were detected,and each pair of primers detected 3-10 alleles with an average of 5.8.The genetic diversity coefficients among the 92 mungbean varieties varied from 0.033 4-1.000 0 with an average of 0.46.Unweighted pair-group method with arithmetic means (UPGMA) clustering analysis showed that these mungbean varieties were clustered into 2 groups at theof genetic similarity coefficient of 0.294 4.SSR fingerprinting database and molecular identification of the 92 mungbean cultivars were established,and it was found that most of the tested varieties,with different SSR fingerprints,which could be molecularly discriminated from each other,could serve as cultivars-specific patterns and as an important basis for cultivar identification.Conclusions:There was a high level of genetic diversity among mungbean germplasms from Inner Mongolia,but a certain degree of inbreeding also existed.Therefore,further efforts are needed to develop new mungbean breeding materials.SSR fingerprinting and molecular identification using fluorescent labeled SSR marker technique is of great significance for genetic authentication,identity validation,traceability management and origin protection of mungbean varieties.关键词
SSR荧光标记/绿豆/遗传多样性分析/指纹图谱构建Key words
fluorescent labeled SSR markers/mungbean varieties/genetic diversity analysis/fingerprint construction分类
农业科技引用本文复制引用
赵雅楠,王颖,张东杰,王丽侠,佐兆杭..内蒙古地区绿豆品种遗传多样性SSR分析及DNA指纹图谱构建[J].食品科学,2017,38(16):43-50,8.基金项目
“十二五”农村领域国家科技计划项目(2012BAD34B02) （2012BAD34B02）
黑龙江省应用技术与开发重大项目(GA14B104) （GA14B104）
大庆科技局创新项目(sjh-2013-65) （sjh-2013-65）
黑龙江省研究生创新科研项目(YJSCX2017-Y50) （YJSCX2017-Y50）
