食品科学2017，Vol.38Issue(16)：140-144,5.DOI:10.7506/spkx1002-6630-201716022
近红外漫反射光谱法快速检测谷子蛋白质和淀粉含量
Application of Near Infrared Diffuse Reflectance Spectroscopy in Rapid Detection of Crude Protein and Starch in Foxtail Millet
摘要
Abstract
This study aimed to establish a near infrared (NIR) diffuse reflectance spectrometry method for measuring the contents of crude protein and starch in foxtail millet for providing a fast,simple and non-destructive analytical method for the identification and screening of foxtail millet germplasm resources.For this purpose,a total of 191 samples of a foxtail millet core collection in Shanxi province were measured using NIR diffuse reflectance spectroscopy.The results showed that with the spectral preprocessing method of first derivative + vector normalization,calibration models were established to predict the contents of crude protein and starch in foxtail millet.The corresponding coefficients of determination for calibration (R~) were 0.977 0 and 0.907 3,and the root mean square errors of cross-validation (RMSECV) were 0.203％ and 0.466％,the coefficients of determination for extemal validation (R2val) were 0.989 6 and 0.977 2,and the root mean square errors of prediction (RMSEP) were 0.225％ and 0.368％,respectively.For the detection of crude protein and starch foxtail millet,no significant differences were seen between chemical analysis and NIR measurement,but the results obtained with NIR measurement were more accurate and reliable.NIR diffuse reflectance spectrometry technology can be useful for the detection of crude protein and starch in foxtail millet.关键词
谷子/近红外漫反射光谱/蛋白质/淀粉Key words
foxtail millet/near infrared (NIR) diffuse reflectance spectrometry/crude protein/starch分类
农业科技引用本文复制引用
田翔,刘思辰,王海岗,秦慧彬,乔治军..近红外漫反射光谱法快速检测谷子蛋白质和淀粉含量[J].食品科学,2017,38(16):140-144,5.基金项目
国家谷子高粱产业技术体系谷子糜子生理岗位项目(CARS-06-13.5-A16) （CARS-06-13.5-A16）
国家农作物种质资源平台山西作物子平台项目(NICGR2016-02) （NICGR2016-02）
山西省农作物种质资源鉴定评价研究项目(2017ZZCX-17) （2017ZZCX-17）
山西省省级财政支农专项(2016ZYZX41) （2016ZYZX41）
