现代食品科技2017，Vol.33Issue(8)：1-6,29,7.DOI:10.13982/j.mfst.1673-9078.2017.8.001
竹节参总皂苷通过调节miR-34a/PPARα干预小鼠脂肪肝的作用研究
Protective Effects of Panax japonicus Saponins on Non-alcoholic Fatty Liver Disease through MiR-34a and PPARα Expression Regulation
摘要
Abstract
The protective effects of total saponins from Panax japonicus (TSPJ) on non-alcoholic fatty liver disease (NAFLD) in mice were investigated.A mouse model of NAFLD was established by feeding a high-fat,high-fructose diet,with simultaneous gavage administration of TSPJ (50 mg/kg and 100 mg/kg) to the TSPJ group for 3 weeks.Serum-and liver-related indicators were monitored;histopathologic changes in liver were observed using hematoxylin-eosin (HE) and oil red O staining.Real-time polymerase chain reaction (RT-PCR) and western blotting were utilized for the detection of transcriptional levels of miRNA and related genes in liver tissue and the expression level of peroxisome proliferator-activated receptor (PPAR) α,respectively.The levels of serum alanine aminotransferase (ALl) and liver triglyceride (TG) in the model group were higher than those in the normal group.HE staining and oil red O staining showed fatty degeneration and fat deposition in the liver tissue,suggesting that a successful NAFLD model had been established.The results of RT-PCR and western blot analysis revealed that the expression levels of microRNA (miR)-34a,sterol regulatory element binding protein (SREBP)-1c,and fatty acid synthase (FASN) were significantly increased.Compared with the fatty liver group,the TSPJ treatment group showed significantly improved histopathologic changes in liver,reduced fat deposition,and reduced ALT levels.Therefore,intervention of the total saponins of Panaxjaponicus on fatty liver in mice was realized by blocking miR-34a,targeting PPARα signaling pathways.关键词
竹节参总皂苷/非酒精性脂肪肝病/miR-34a/PPARα/SREBP-1cKey words
total saponins of Panax japonicus/non-alcoholic fatty liver diseases/microRNA-34a/peroxisome proliferator-activated receptor alpha/sterol regulatory element binding protein-1c引用本文复制引用
熊海容,吴利春,段丽,张长城,袁丁,刘朝奇..竹节参总皂苷通过调节miR-34a/PPARα干预小鼠脂肪肝的作用研究[J].现代食品科技,2017,33(8):1-6,29,7.基金项目
国家自然科学基金面上项目(81673675) （81673675）
