现代食品科技2017，Vol.33Issue(8)：130-140,11.DOI:10.13982/j.mfst.1673-9078.2017.8.020
微型DPPH、ABTS和FRAP法动态监测从红酒中分离得到的不同种类多酚化合物的抗氧化活性
A Micro-model of DPPH, ABTS, and FRAP for Dynamic Monitoring of the Antioxidant Activity of Different Types of Polyphenols Isolated from Red Wine
摘要
Abstract
The optimal reaction time and the antioxidant activity of different types of polyphenols isolated from red wine were determined using a micro-model of 2,2-diphenyl-l-picrylhydrazyl (DPPH),2,20-Azino-bis (3-ethylben zothiazoline-6-sulfonic acid) diammonium salt (ABTS),and ferric reduction ability plasma (FRAP) assays.Using Trolox and vitamin ℃ as controls,the reaction processes of different types of red wine polyphenols in DPPH,ABTS,and FRAP systems were dynamically monitored,and the antioxidant activity of red wine polyphenols at different concentrations were calculated.The results showed that the optimal reaction times of DPPH,ABTS,and FRAP were 100 min,240 min,and 370 min,respectively.Different types of red wine polyphenols were similar to the antioxidants,and their antioxidant activity showed a dose-dependent relationship with concentration.The correlation analysis suggested that epicatechin,polymers,oligomers,and anthocyanins were the main components affecting the antioxidant activity of red wine and could be determined as indicators for red wine quality control.The micro-model ofDPPH,ABTS,and FRAP established in this study was simple and reproducible,and demonstrated low sample consumption.关键词
DPPH/ABTS/FRAP/红酒多酚/抗氧化活性Key words
2,2-diphenyl-l-picrylhydrazyl (DPPH)/2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)/ferric reducing ability of plasma (FRAP)/red wine polyphenols/antioxidant activity引用本文复制引用
李媛媛,李灵犀,崔艳,孙宝山..微型DPPH、ABTS和FRAP法动态监测从红酒中分离得到的不同种类多酚化合物的抗氧化活性[J].现代食品科技,2017,33(8):130-140,11.基金项目
辽宁省科技厅引进海外研发团队专项资金(辽外专2011-5号) （辽外专2011-5号）
