现代食品科技2017，Vol.33Issue(8)：269-275,7.DOI:10.13982/j.mfst.1673-9078.2017.8.039
实时荧光PCR法快速鉴别狐狸貉子肉源性成分研究
Rapid Detection of Alopex lagopus-and Nyctereutes procyonoides-derived Constituents of Meat Products Using RT-PCR
摘要
Abstract
Alopex lagopus-and Nyctereutes procyonoides-specific primers and TaqMan probes were designed based on the conserved sequences in the mitochondrial genome of the Alopex lagopus and the displacement-1oop (D-loop) region of Nyctereutes procyonoides.A real-time polymerase chain reaction (RT-PCR)-based method for the determination of Alopex lagopus-and Nyctereutes procyonoides-derived constituents of commercial meat products was developed in this study.The established RT-PCR method was evaluated for specificity,sensitivity,and linearity;the method showed good specificity and sensitivity,and the limits of detection for Alopex lagopus DNA and Nyctereutes procyonoides DNA were 0.5 pg/μL and 5 pg/μL,respectively.In summary,this RT-PCR method can be used for the identification of Alopex lagopus-and Nyctereutesprocyonoides-derived constituents in commercial samples.关键词
实时荧光聚合酶链式反应/狐狸源性成分/貉子源性成分/肉类掺假Key words
real-time polymerase chain reaction/Alopex lagopus-derived constituents/Nyctereutes procyonoides-derived constituents/meat adulteration引用本文复制引用
章晶晶,杜利强,李永艳,李顺才,齐艳玲,李永波,张岩,周巍..实时荧光PCR法快速鉴别狐狸貉子肉源性成分研究[J].现代食品科技,2017,33(8):269-275,7.基金项目
“十二五”国家科技支撑计划项目(2015BAK36B03) （2015BAK36B03）
河北省科技厅重点研发计划项目(16275502D) （16275502D）
河北省高等学校科学技术研究青年基金项目(QN2014159) （QN2014159）
