食品与发酵工业2017，Vol.43Issue(8)：22-26,5.DOI:10.13995/j.cnki.11-1802/ts.013991
磷酸转移酶系统关键基因敲除对Klebsiella pneumoniae产1,3-丙二醇的影响
The effects of knocking out key genes in phosphotransferase system on 1,3-propanediol production of Klebsiella pneumoniae
陆竞争 1任顺利 2诸葛斌 1陆信曜 2宗红 1方慧英 2宋健1
作者信息
- 1. 江南大学,糖化学与生物技术教育部重点实验室,江苏无锡,214122
- 2. 江南大学,工业生物技术教育部重点实验室,工业微生物研究中心,江苏无锡,214122
- 折叠
摘要
Abstract
During production of 1,3-propanediol from glycerol by Klebsiella pneumoniae with glucose as co-substrate,glucose has the priority of being used for accumulated biomass and byproducts due to the carbon catabolism repression (CCR),which results in an obstacle on 1,3-propanediol production.Based on the phosphotransferase system (PTS) involved in glucose specific transferance and corresponding carbon metabolic regulation,ptsG gene encoding E Ⅱ BCGlc and crr gene encoding EⅡ AGlc were respectively knocked out by Red disruption system in this study.The effects of ptsG,crr-deficient PTS on cell growth,1,3-propanediol production,and byproducts accumulation were examined.It was observed that after knocking out ptsG and crr genes,the yields of glycerol increased by 26.2％,42.7％ compared with the parent strains,respectively.A yield of 23.1 g/L 1,3-propanediol by K.pneumoniae △crr was achieved with increase of 35.8％.The results above demonstrated that modification of PTS by disruption of ptsG and crr genes could improve the yield of glycerol and enhance the 1,3-propanediol production.关键词
crr/ptsG/磷酸转移酶系统/克雷伯氏菌/1,3-丙二醇Key words
crr/ptsG/phosphotransferase system (PTS)/Klebsiella pneumoniae/1,3-propanediol引用本文复制引用
陆竞争,任顺利,诸葛斌,陆信曜,宗红,方慧英,宋健..磷酸转移酶系统关键基因敲除对Klebsiella pneumoniae产1,3-丙二醇的影响[J].食品与发酵工业,2017,43(8):22-26,5.
