食品与发酵工业2017，Vol.43Issue(8)：127-133,7.DOI:10.13995/j.cnki.11-1802/ts.013517
以豆粕为基质的植物乳杆菌固态发酵菌剂的制备
Optimization of solid-state culture conditions for Lactobacillus plantarum agent utilizing soybean meal as nitrogen source
摘要
Abstract
Research herein was focused on the best condition for preparation of Lactobacillus plantarum agent with soybean meal containing 2％ glucose.The number of viable cells after drying was used as an indicator to investigate the production process.The production process was optimized through one-factor experiment combined with response surface methodology.After fermentation,content of crude protein,acid soluble protein,free amino acids,trypsin inhibitor,urease,and phytic acid,as well as protein digestibility were determined.Results showed that Lactobacillus plantarum achieved the highest biomass after incubation at 30.6 ℃ for 46.40 h,with substrate to water ratio of 1∶0.61 and inoculum concentration of 4.45％.The content of crude protein,acid soluble protein,and total free amino acids,and protein digestibility were increased 4.55％,1.24％,50.47 mg/g and 4.21％ respectively.The content of trypsin inhibitor,urease,and phytic acid were decreased 94.59％,91.64％,and 30.22％,respectively.The number of Lactobacillus plantarum could reach 9.88 lg(CFU/g) under the best drying condition of 50℃ hot air for4 h.关键词
植物乳杆菌/豆粕/固态发酵/Box-Behnken试验Key words
Lactobacillus plantarum/soybean meal/solid-state fermentation/Box-Behnken experimental design引用本文复制引用
王彦萍,熊涛,王浩,彭珍,黄涛..以豆粕为基质的植物乳杆菌固态发酵菌剂的制备[J].食品与发酵工业,2017,43(8):127-133,7.基金项目
江西省主要学科学术和技术带头人培养计划(20133BCB22001) （20133BCB22001）
江西省重大科技专项计划(赣财教字[2014]104号) （赣财教字[2014]104号）
“赣鄱英才555工程”领军人才培养计划(赣才字[2013]2号) （赣才字[2013]2号）
江西省蔬菜产业技术体系岗位专家项目(JX-ARS-06) （JX-ARS-06）
