食品与发酵工业2017，Vol.43Issue(8)：229-232,4.DOI:10.13995/j.cnki.11-1802/ts.12206
高效液相色谱法同时测定发酵液中胡椒醛、胡椒酸以及黄樟油素
Determination of piperonal, piperonylic acid and safrole in fermentation broth by high performance liquid chromatography
赵明涛 1郑璞 1邢晨光 2刘思琴 1赵希景 2陈鹏程1
作者信息
- 1. 江南大学生物工程学院,工业生物技术教育部重点实验室,江苏无锡,214122
- 2. 厦门欧米克生物科技有限公司,生物工程试验室,福建厦门,361000
- 折叠
摘要
Abstract
A high performance liquid chromatography method was developed for the determination of piperonal,piperonylic acid and safrole in fermentation broth.The separation was performed on Amethyst C18-H C18 column (250mm × 4.6 mm,5μm) using gradient elution,the mobile phase A was acetonitrile and B was 0.1％ formic acid solution.The column temperature was chosen for 30 ℃ and the detection wave length was 270 nm.It was found that piperonal,piperonylic acid and substrate safrole could be well separated in the conditions,which showed that average recoveries were within the range of 98.0％-101.0％,and the relative standard deviations ranging from 0.5％ to 2.0％ in samples.This method was also applied for the determination of fermentation broth of Serratia liquefaciens M2016170,which contained 182 mg/L piperonal and 9.8 mg/L piperonylic acid.关键词
高效液相色谱/胡椒醛/胡椒酸/液化沙雷氏菌/发酵液Key words
high performance liquid chromatography/piperonal/piperonylic acid/Serratia liquefaciens/fermentation broth引用本文复制引用
赵明涛,郑璞,邢晨光,刘思琴,赵希景,陈鹏程..高效液相色谱法同时测定发酵液中胡椒醛、胡椒酸以及黄樟油素[J].食品与发酵工业,2017,43(8):229-232,4.
