中国食物与营养2017，Vol.23Issue(8)：33-36,4.
HPLC法测定南瓜中β-胡萝卜素
Determination on β-carotene in Fresh Pumpkin by HPLC Method
摘要
Abstract
[Objective] A High performance liquid chromatography (HPLC) method was established for determination on the contents of β-carotene in fresh pumpkin and the contents of β-carotene in 12 pumpkins were determined.[Method] A Eclipse XDB-C18 colunm (4.6 mm ×250mm,5 μm) was used to separate the compound.The Mobile phase was consisted by a mixture of water and acetone and the detection wavelength was carried out 450 nm.[Result] The β-carotene was separated within 35 min,and the method was of good repeatability (RSD =5.11％),high precision (RSD =0.70％),good stability (RSD =2.73％),and the recovery rate was accurate and reliable (RSD =2.06％).The contents of β-carotene in 12 Pumpkins were different (8.9 ～94.4 μg/g).Cucurbita maxima 16296-1 had the the highest content of beta carotene,followed by Cucurbita moschata 9132,Cucurbita maxima 16291-2 and 16167-8,the amounts of β-carotene in these pumpkin were closer or bigger 76μg/g,Cucurbita maxima 16185-6 had the lowest β-carotene content.[Conclusion] A accurate and reliable method of high performance liquid chromatography (HPLC) was established to detect the β-carotene in pumpkin and the content of β-carotene in 12 pumpkin samples had the big differences.关键词
南瓜/β-胡萝卜素/HPLCKey words
pumpkin/β-carotene/HPLC引用本文复制引用
邓俊琳,朱永清,刘小俊,杨宏,梁根云,陈建,夏陈,张盈娇,李娟..HPLC法测定南瓜中β-胡萝卜素[J].中国食物与营养,2017,23(8):33-36,4.基金项目
公益性行业(农业)科研专项经费(项目编号:201303112) （农业）
国家现代农业产业技术体系专项资金资助(项目编号:CARS-23-G32). （项目编号:CARS-23-G32）
