食品科学2017，Vol.38Issue(17)：1-7,7.DOI:10.7506/spkx1002-6630-201717001
双歧杆菌细菌素Bifidocin A对金黄色葡萄球菌的抑菌作用及其机制
Antibacterial Activity and Mechanism of Bifidocin A against Staphylococcus aureus
摘要
Abstract
Bifidocin A, produced by Bifidobacterium animalis BB04, is a novel bacteriocin with antimicrobial activity against a wide range of foodborne bacteria. The objective of this study was to investigate the antibacterial activity and mechanism of action of bifidocin A against Staphylococcus aureus. The minimum inhibitory concentration (MIC) of bifidocin A for Staphylococcus aureus CVCC 26112 was 0.058 μg/mL. Time-kill assays showed that bifidocin A effectively inhibited the growth of Staphylococcus aureus CVCC 26112 in a time- and concentration-dependent manner. Results also suggested that bifidocin A exerted its anti-Staphylococcus aureus effect through the dissipation of the cytoplasmic membrane proton motive force (PMF), increased membrane permeability, and the formation of cell membrane pores, leading to the destruction of membrane integrity and ultimately complete disintegration of the cells.关键词
细菌素/双歧杆菌/金黄色葡萄球菌/抑菌活性/抑菌机制Key words
bacteriocin/Bifidobacterium spp./Staphylococcus aureus/antibacterial activity/antibacterial mechanism分类
轻工纺织引用本文复制引用
刘国荣,郜亚昆,王欣,刘玉洁,曹季林,王成涛..双歧杆菌细菌素Bifidocin A对金黄色葡萄球菌的抑菌作用及其机制[J].食品科学,2017,38(17):1-7,7.基金项目
国家自然科学基金面上项目(31671832) （31671832）
北京市属高校高水平教师队伍建设支持计划青年拔尖人才培育计划项目(CIT&TCD201704034) （CIT&TCD201704034）
国家自然科学基金青年科学基金项目(31201407) （31201407）
