食品科学2017，Vol.38Issue(17)：8-13,6.DOI:10.7506/spkx1002-6630-201717002
体外模拟消化对车前子来源毛蕊花苷抑制黄嘌呤氧化酶活性的影响
Effects of in Vitro Simulated Degradation on the Xanthine Oxidase Inhibitory Activity of Verbascoside from Plantago asiatica L.
摘要
Abstract
In this study, the effects of different stages of in vitro simulated digestion on the stability of Plantago asiatica L. seed verbascoside (PSV) were explored. It was found that the concentration of PSV in all simulated digestion systems was reduced after digestion for 4 h, and the highest reduction of (71.26 ± 0.96)% was observed in the gut bacterial culture, followed by the simulated gastrointestinal digestion system ((28.46 ± 0.50)%). After simulated digestion, xanthine oxidase inhibitory activity in various simulated digestion systems increased, in particular in the gut bacterial culture (P < 0.05), followed by the simulated gastrointestinal digestion system. The aglycone metabolites of verbascoside, caffeic acid and hydroxytyrosol, were detected from all simulated digestion systems by LC-MS. The in vitro xanthine oxidase inhibitory activity of caffeic acid and hydroxytyrosol was higher than that of verbascoside. The degradation of verbascoside into xanthine oxidase inhibitory metabolites is likely to be one of the effective ways to exert its physiological activity in vivo.关键词
毛蕊花苷/模拟消化/黄嘌呤氧化酶/体外抑制作用/苷元Key words
verbascoside/simulated digestion/xanthine oxidase/in vitro inhibition activity/aglycone分类
轻工纺织引用本文复制引用
万茵,张阿惜,陈雪洋,陈明,余迎利,夏冬华,付桂明..体外模拟消化对车前子来源毛蕊花苷抑制黄嘌呤氧化酶活性的影响[J].食品科学,2017,38(17):8-13,6.基金项目
国家自然科学基金地区科学基金项目(31160316) （31160316）
