食品科学2017，Vol.38Issue(17)：14-19,6.DOI:10.7506/spkx1002-6630-201717003
γ射线联合肉桂精油对腐败希瓦氏菌的抑制机制
Antimicrobial Mechanism of Combination of γ Irradiation and Cinnamon Essential Oil against Shewanella putrefaciens
摘要
Abstract
The combined antibacterial effect of γ irradiation and cinnamon essential oil (CEO) against Shewanella putrefaciens was evaluated and the underlying mechanism was investigated. The irradiation dose required to reduce total colony number to 10% of the initial level (D10) was reduced and the relative irradiation sensitivity was increased with increased concentration of CEO. The combination of γ irradiation and CEO was more effective in inhibiting the growth of S. putrefaciens than each alone. The combination treatment exhibited an obvious negative effect on cell morphology, intracellular ATPand pH, membrane proteins and fatty acids in S. putrefaciens. The combination resulted in a reduction of 40 kD subunits and an increase of 18 kD subunits in membrane proteins, as well as a significant reduction of C14:0, C16:0, C16:1, C17:1, and C18:1 in membrane lipids (P < 0.05). The mechanism for the synergism was due to the potential of γ irradiation to increase the destructive effect of CEO on the cell membrane of S. putrefaciens thus causing its death.关键词
辐照/肉桂精油/腐败希瓦氏菌/联合作用/抑菌机理Key words
irradiation/cinnamon essential oil/Shewanella putrefaciens/synergistic effect/antimicrobial mechanism分类
轻工纺织引用本文复制引用
吕飞,徐静,魏倩倩,高飞,周绪霞,丁玉庭,刘璘..γ射线联合肉桂精油对腐败希瓦氏菌的抑制机制[J].食品科学,2017,38(17):14-19,6.基金项目
国家自然科学基金青年科学基金项目(31301579) （31301579）
