食品科学2017，Vol.38Issue(17)：36-41,6.DOI:10.7506/spkx1002-6630-201717007
新疆莎车1号扁桃仁2S-清蛋白的纯化及特性研究
Purification and Characteristics of 2S-Albumin in Shache No. 1 Almond from Xinjiang
摘要
Abstract
Albumin is the major protein in almond. In this experiment, we purified and structurally characterized 2S-albumin from Shache No.1 almond (SC-1) from Xinjiang. Crude albumin extract was obtained from almond by repeated precipitation with organic solvents and then fractionated chromatographically on Q Sepharose FF to obtain 2S-albumin. By HPLC-MS analysis, the accurate molecular mass of 2S-albumin was determined to be 31.40 kD, which was separated into two bands (18.0 and 19.5 kD) in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), indicating that it was composed of two distinct subunits connected through disulfide bonds. In 2S-albumin, the contents of glutamic acid, aspartic acid and arginine residues were relatively high, and its dominant secondary structure was β-sheet (65.9%). The carbonyl group content of 2S-albumin was 4.45 nmol/mg, the active thiol group content was 39.95 μmol/g, the total thiol group content was 57.11 μmol/g, the surface hydrophobicity was 23.45 μg, and the denaturation temperature was 43.3 ℃. These results can provide a theoretical support for the development and utilization of Xinjiang grown almond.关键词
扁桃仁/2S-清蛋白/纯化/结构特性Key words
almond/2S-albumin/purification/structure characteristics分类
轻工纺织引用本文复制引用
李述刚,陆健康,王萍,马美湖..新疆莎车1号扁桃仁2S-清蛋白的纯化及特性研究[J].食品科学,2017,38(17):36-41,6.基金项目
国家自然科学基金青年科学基金项目(31401644) （31401644）
兵团中青年创新领军人才项目(2016BC001) （2016BC001）
