食品科学2017，Vol.38Issue(17)：42-46,5.DOI:10.7506/spkx1002-6630-201717008
大豆蛋白肽-酪蛋白非磷酸肽组装产物的荧光光谱分析及抗氧化性研究
Fluorescence Spectral Analysis and Antioxidant Effect of Soybean Ploypeptide-Casein Non-Phosphorylated Peptide Assembly
摘要
Abstract
The fluorescence spectra and antioxidant properties of casein non-phosphopeptides-soybean peptide complex (CNPSPC) prepared under different pH conditions were analyzed. The results showed that with the increase of pH, the content of surface-exposed Trp residues buried in hydrophobic environment in CNPSPC was became lower than in the raw protein, and the microenvironment polarity of Trp residues was decreased. The results of thioflavin T (ThT) fluorescence assay showed that the fluorescence intensity of CNPSPC was maximum at pH 6.0, which represented β-sheet was the dominant secondary structure of CNPSPC. The microenvironment of Trp residues was changed by the assembly, thereby changing the molecular conformation of CNPSPC. Compared with casein non-phosphopeptides (CNPP) and soybean peptide (SP), the antioxidant capacity of CNPSPC was improved. The scavenging activities of CNPSPC against 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion and hydrolxyl radicals were maximum at pH 6.0, which increased by 3.34, 1.12 and 1.08 times compared to soybean protein respectively.关键词
大豆蛋白肽/酪蛋白非磷酸肽/组装/荧光光谱/抗氧化性Key words
soybean polypeptide/casein non-phosphorylated peptide/assembly/fluorescence spectrum/antioxidant activity分类
轻工纺织引用本文复制引用
张娜,郭庆启,黄文秀,石彦国..大豆蛋白肽-酪蛋白非磷酸肽组装产物的荧光光谱分析及抗氧化性研究[J].食品科学,2017,38(17):42-46,5.基金项目
"十三五"国家重点研发计划重点专项(2016YFD0400400) （2016YFD0400400）
黑龙江省博士后科研启动项目(LBH-Q15073) （LBH-Q15073）
哈尔滨市科技局科技创新人才研究专项资金项目(2016RAQXJ146) （2016RAQXJ146）
中国食品科学技术学会食品科技基金项目(2016-002) （2016-002）
国家自然科学基金青年科学基金项目(31301602) （31301602）
黑龙江省应用技术研究与开发计划项目(GC13B215 （）
GA14B201) （）
