食品科学2017，Vol.38Issue(17)：126-131,6.DOI:10.7506/spkx1002-6630-201717021
远志多糖的分离纯化、结构特征及生物活性
Purification and Structural Characterization of Polysaccharide from Polygala tenuifolia and Its Biological Activity
摘要
Abstract
Objective: To isolate and purify a polysaccharide from dried roots of Polygala tenuifolia and to determine its physicochemical properties and biological activity. Methods: The purified polysaccharide was obtained by ultrasonic-assisted extraction, alcohol fractional precipitation, deproteinization, decolorization, dialysis, DEAE-cellulose 52 and Sephadex G-100 gel column chromatography. The purity of the final purified product was determined by UV-visible spectroscopy, spin spectrophotometry and gel filtration chromatography (GPC). The relative molecular mass of the polysaccharide was determined by high performance gel permeation chromatography, the monosaccharide composition was analyzed by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), the antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl free radical scavenging assays, and the hypoglycemic activity was evaluated by α-glucosidase-inhibiting activity. Results: A homogeneous polysaccharide (PTP-1) was obtained. The relative molecular mass of PTP-1 was 4.62 × 104. Its monosaccharide composition was composed of galactose, glucose and mannose with a molar ratio of 3.92:1.00:2.08. The polysaccharide showed DPPH and hydroxyl radical scavenging capacity in a dose-dependent manner in a certain concentration range, with IC50 of 0.35 and 0.51 mg/mL, respectively. In addition, it was able to significantly inhibit α-glucosidase activity with IC50 of 0.52 mg/mL. Conclusion:PTP-1 is a pure polysaccharide with antioxidant and hypoglycemic activity.关键词
远志/多糖/理化性质/抗氧化活性/降血糖活性Key words
Polygala tenuifolia/polysaccharide/physicochemical property/antioxidant activity/hypoglycemic activity分类
轻工纺织引用本文复制引用
景永帅,张丹参,吴兰芳,郑玉光..远志多糖的分离纯化、结构特征及生物活性[J].食品科学,2017,38(17):126-131,6.基金项目
河北省自然科学基金项目(H2016208059 （）
H2016208058 （）
H2017423023) （）
河北省教育厅高等学校科学技术研究项目(QN2016187 （）
QN2016099 （）
ZD2016009) （）
河北省食药监局食品药品安全科技项目(QN2015016 （）
QN2016013) （）
