食品科学2017，Vol.38Issue(17)：184-189,6.DOI:10.7506/spkx1002-6630-201717030
日粮补充L-亮氨酸对超早期断奶宫内发育迟缓仔猪肠道免疫功能的影响
Effect of Dietary L-Leucine Supplementation on Intestinal Immune Function in Ultra-Early Weaned Piglets with Intrauterine Growth Retardation
摘要
Abstract
Objective: To ascertain the effect of dietary leucine supplementation on intestinal immune function in ultra-early weaned piglets suffering from intrauterine growth retardation (IUGR). Methods: Sixteen normal birth weight (NBW) piglets weaned at 14 days of age and sixteen IUGR newborn piglets were selected according to their birth weight. All the piglets were randomly divided into four groups with 8 replicates in each group, and fed control diet alone or supplemented with 0.35% L-leucine for 21 days, respectively. Results: Compared with NBW piglets, IUGR piglets showed significantly lower lysozyme activity and complement-3 (C3) level in the serum (P < 0.05), a significant reduction in the number of goblet cells (GC) and intraepithelial lymphocytes (IEL) in the jejunum and ileum (P < 0.05) and interleukin-10 (IL-10) content in the ileum, and a significant increase intumor necrosis factor-α (TNF-α) content in the ileum (P < 0.05). In addition, IUGR piglets had significantly increased TNF-α mRNA expression but significantly decreased IL-10 mRNA expression (P < 0.05). Dietary supplementation with 0.35% L-leucine significantly increased lysozyme activity and the levels of C3 and C4 in the serum, the numbers of GC in the ileum and IEL in the duodenum and jejunum, IL-10 content in the ileum, and the mRNA expression of IL-2 and IL-10 in the ileum of IUGR piglets (P < 0.05). Conclusion: IUGR could induce intestinal mucosal inflammatory response and hinder intestinal growth and development in early weaned piglets. L-Leucine could effectively alleviate intestinal mucosal injury caused by IUGR, and improve intestinal immune function in piglets.关键词
L-亮氨酸/宫内发育迟缓/肠道/免疫功能/早期断奶仔猪Key words
L-leucine/intrauterine growth retardation (IUGR)/intestine/immune function/early weaned piglets分类
轻工纺织引用本文复制引用
黄强,徐稳,何进田,白凯文,苏伟鹏,周乐,张莉莉,王恬..日粮补充L-亮氨酸对超早期断奶宫内发育迟缓仔猪肠道免疫功能的影响[J].食品科学,2017,38(17):184-189,6.基金项目
国家重点基础研究发展计划(973计划)项目(2012CB124703) （973计划）
