食品科学2017，Vol.38Issue(17)：203-209,7.DOI:10.7506/spkx1002-6630-201717033
普洱茶茶色素对HepG2细胞脂质代谢的影响及作用机理
Effect and Mechanism of Pigments from Pu-erh Tea on Lipid Metabolism in HepG2 Cell Model
摘要
Abstract
The contents of triglyceride (TG) and total cholesterol (TG), the mRNA expression level of fatty acid synthase (FAS), sterol regulatory element binding protein 1c (SREBP-1c), cholesterol 7α-hydroxylase (CYP7A1) and ATP binding cassette transporter A1 (ABCA1), and the protein expression level of phospho-AMP-activated protein kinase (p-AMPK) were investigated for studying the effects of pigments from Pu-erh tea on lipid metabolism in HepG2 cell line cultured with normal or oleic acid enriched medium. The results showed that pigments from Pu-erh tea significantly decreased the contents of TG and TC in HepG2 cells cultured in oleic acid-enriched medium in a dose-dependent manner, although having no significant effect on HepG2 cells cultured in normal medium. The mRNA expression levels of FAS and SREBP-1c were significantly down-regulated, and the mRNA level of ABCA1 was significantly up-regulated, the mRNA expression level of CYP7A1 showed an increased tendency and the protein expression level of p-AMPK was significantly up-regulated after the treatment with pigments from Pu-erh tea for 24 hours in HepG2 cells cultured in oleic acid-enriched medium (P < 0.05). Therefore, pigments from Pu-erh tea could improve lipid metabolism in HepG2 cells cultured in oleic acid-enriched medium by regulating the mRNA levels of enzymes and transcription factors.关键词
HepG2/脂质代谢/普洱茶/色素Key words
HepG2/lipid metabolism/Pu-erh tea/pigments分类
医药卫生引用本文复制引用
陈亚蓝,白晓丽,李长文,王雪青,王怡雯,郑子晴,叶美霞,付芳芳,赵培,宋文军,王素英..普洱茶茶色素对HepG2细胞脂质代谢的影响及作用机理[J].食品科学,2017,38(17):203-209,7.基金项目
天津市高等学校创新团队建设规划资助项目(TD12-5049) （TD12-5049）
天津市高校科技发展基金计划项目(20120603) （20120603）
天津商业大学大学生研究训练计划(SRT)基金项目 （SRT）
