食品科学2017，Vol.38Issue(17)：210-215,6.DOI:10.7506/spkx1002-6630-201717034
灵芝多糖对CCl4诱导的急性肝损伤小鼠的抗炎和保肝活性
Anti-Inflammatory and Hepatoprotective Effects of Ganoderma lucidum Polysaccharides on Carbon Tetrachloride-Induced Acute Liver Injury in Mice
摘要
Abstract
Objective: To study the anti-inflammatory and hepatoprotective effects of Ganoderma lucidum polysaccharides (GLPS) in mice with acute liver injury induced by carbon tetrachloride (CCl4), and to explore the possible mechanism. Methods: Mice were randomly divided into six groups: normal control group, acute liver injury model group (0.1% (V/V) CCl4, 20 mL/(kg bw·d), low, medium and high-dose GLPS groups (50, 100 and 150 mg/(kg bw·d)), and bifendate group (100 mg/(kg bw·d)). After administration for 7 continuous days, the body weight, liver weight, liver index, alanine transferase (ALT) and aspartate transferase (AST) activities, and total bilirubin (TBIL) level in serum were detected, and the malondialdehyde (MDA) and glutathione-SH (GSH) levels, and nitric oxide synthase (NOS) activity in liver tissue were also analyzed. The levels of interleukin (IL)-1β, IL-18, IL-6 and TNF-α in serum and IL-1β in liver tissue were analyzed by commercial enzyme linked immunosorbent assay (ELISA) kits. The protein expression levels of NLRP3, ASC and caspase-1 in liver tissue were evaluated by Western blot. Results: Compared with the normal control group, CCl4 significantly increased liver weight and liver index, improved serum ALT and AST activities, enhanced the levels of TBIL, IL-1β,IL-18, IL-6 and TNF-α in serum and MDA, IL-1β and NOS in liver tissue, up-regulated the protein expression of NLRP3, ASC and caspase-1 in liver tissue, and remarkably reduced GSH level in liver tissue in the model group. Compared with the acute liver injury model group, GLPS and bifendate notably inhibited ALT and AST activities, decreased liver weight and index, as well as TBIL, IL-1β, IL-18, IL-6 and TNF-α in serum and MDA, IL-1β and NOS in liver tissue, remarkably enhanced GSH levels, down-regulated the protein expression NLRP3, ASC and caspase-1 in liver tissue, notably ameliorated the morphological changes of liver tissue. Conclusion: GLPS has a significant anti-inflammatory and hepatoprotective effect on CCl4-induced liver injury in mice. The possible mechanism may be related to the inhibition of free radical lipid peroxidation, NOS activity and the activation of liver inflammatory factors.关键词
灵芝多糖/肝损伤/抗炎/保肝/联苯双酯Key words
Ganoderma lucidum polysaccharides/liver injury/anti-inflammatory/hepatoprotective/bifendate分类
医药卫生引用本文复制引用
陈玉胜,陈全战..灵芝多糖对CCl4诱导的急性肝损伤小鼠的抗炎和保肝活性[J].食品科学,2017,38(17):210-215,6.基金项目
南京市重点学科基金项目(NJZDXK201407) （NJZDXK201407）
