食品科学2017，Vol.38Issue(17)：273-278,6.DOI:10.7506/spkx1002-6630-201717044
臭氧对葡萄灰霉病的抑制效果
Effect of Ozone on Controlling Gray Mold in Grapes
摘要
Abstract
The aim of this study is to provide theoretical support for reducing the loss of grapes caused by Botrytis cinerea during storage. Botrytis cinerea was exposed to ozone at different concentrations (0, 30, 60 and 90 μL/L) for different times (5, 10 and 15 min), and then stored at (20 ± 1) ℃. The mycelial diameter, the formation of Botrytis cinerea spores and plasma membrane integrity were analyzed to explore the inhibitory effect of ozone on Botrytis cinerea. In a second experiment, postharvest 'Red Globe' grapes were inoculated with Botrytis cinerea and exposed to four different concentrations of ozone for 15 min, and then stored at (20 ± 1) ℃ for 5 days. The incidence, lesion size and related enzymes activities were measured to evaluate the inhibitory effect of ozone on controlling gray mold in postharvest grapes. The results indicated that compared with the control group, the growth of Botrytis cinerea was inhibited by ozone but the effect decreased gradually over storage time. Ozone could concentration-dependently and time-dependently damage the integrity of the plasma membrane of Botrytis cinerea and reduce conidia germination. On the other hand, the growth of Botrytis cinerea in grapes was strongly inhibited by ozone at concentrations of 60 and 90 μL/L. Meanwhile, ozone could inhibit the accumulation of malondialdehyde (MDA) content and help maintain higher levels of antioxidant enzyme activities. Taken together, this study suggested that ozone was effective against gray mold in postharvest 'Red Globe' grapes by inhibiting the growth of the pathogen and inducing host resistance and the best effect was observed at a concentration of 90 μL/L and 15 min treatment.关键词
臭氧/'红地球'葡萄/灰霉病/贮藏保鲜Key words
ozone/'Red Globe' grapes/gray mold/storage and preservation分类
农业科技引用本文复制引用
郭宇欢,何玲,齐馨,王宇婷,郭赛赛,杨晨茜..臭氧对葡萄灰霉病的抑制效果[J].食品科学,2017,38(17):273-278,6.基金项目
杨凌示范区科技计划项目(2014NY-44) （2014NY-44）
