食品科学2017，Vol.38Issue(18)：1-7,7.DOI:10.7506/spkx1002-6630-201718001
响应面法优化Chlamydomonas sp.212产胞内多糖发酵工艺及其抑菌活性
Optimization of Fermentation Process for the Production of Intracellular Polysaccharide from Chlamydomonas sp. 212 and Its Antimicrobial Activity
摘要
Abstract
Response surface methodology was applied to optimize the fermentation process of intracellular polysaccharide production from Chlamydomonas sp. 212. Then, the antibacterial activity of intracellular polysaccharide was studied by filter paper disc method. The optimum fermentation medium was found to consist of NaNO3305.01 mg/L, NaCl 93.66 mg/L, and NaHCO32.12 g/L. The bacterium grown in the optimized medium yielded 91.1823 mg/L of intracellular polysaccharide, which was increased by 160% compared to that obtained using the unoptimized medium. The intracellular polysaccharide from Chlamydomonas sp. 212 had strong antibacterial activity against Salmonella typhimurium and weak antimicrobial activity against Escherichia coli, Staphylococcus albus, Staphylococcus aureus and Aspergillus niger. This study may provide a theoretical basis for the screening of new natural antimicrobial substances and for the comprehensive utilization of oleaginous microalgae.关键词
衣藻/胞内多糖/响应面法/发酵工艺/抑菌活性Key words
Chlamydomonas sp./intracellular polysaccharide/response surface methodology/fermentation process/antibacterial activity分类
生物科学引用本文复制引用
孙建瑞,符丹丹,赵君峰,王大红,古绍彬..响应面法优化Chlamydomonas sp.212产胞内多糖发酵工艺及其抑菌活性[J].食品科学,2017,38(18):1-7,7.基金项目
国家自然科学基金青年科学基金项目(31401672) （31401672）
河南省科技厅重点攻关项目(162102110056) （162102110056）
河南省高等学校重点科研项目(17B180003) （17B180003）
河南科技大学博士科研启动基金项目(13480054) （13480054）
